{"id":74930,"date":"2021-09-16T14:00:45","date_gmt":"2021-09-16T05:00:45","guid":{"rendered":"https:\/\/www.waseda.jp\/top\/?p=74930"},"modified":"2021-09-16T14:02:18","modified_gmt":"2021-09-16T05:02:18","slug":"%e3%83%8e%e3%83%83%e3%82%af%e3%82%a4%e3%83%b3%e6%96%b0%e6%89%8b%e6%b3%95-bipod-%e3%82%92%e9%96%8b%e7%99%ba","status":"publish","type":"post","link":"https:\/\/www.waseda.jp\/top\/news\/74930","title":{"rendered":"\u30ce\u30c3\u30af\u30a4\u30f3\u65b0\u624b\u6cd5 BiPoD \u3092\u958b\u767a"},"content":{"rendered":"<h1><strong>\u72d9\u3063\u305f\u5834\u6240\u306b\u30c9\u30ca\u30fcDNA\u3092\u633f\u5165\u3059\u308b\u30ce\u30c3\u30af\u30a4\u30f3\u65b0\u624b\u6cd5(BiPoD)\u3092\u958b\u767a<\/strong><\/h1>\n<h2><strong>6%<\/strong><strong>\uff08\u901a\u5e38\u6cd5\uff09\u304b\u308990%\u8d85\uff08\u65b0\u624b\u6cd5\uff09\u306b\u3000\u6b63\u78ba\u6027\u3068\u52b9\u7387\u304c\u5927\u5e45\u306b\u5411\u4e0a<\/strong><\/h2>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-74933\" src=\"https:\/\/www.waseda.jp\/top\/assets\/uploads\/2021\/09\/BiPoD1.png\" alt=\"\" width=\"819\" height=\"315\" srcset=\"https:\/\/www.waseda.jp\/top\/assets\/uploads\/2021\/09\/BiPoD1.png 819w, https:\/\/www.waseda.jp\/top\/assets\/uploads\/2021\/09\/BiPoD1-610x235.png 610w, https:\/\/www.waseda.jp\/top\/assets\/uploads\/2021\/09\/BiPoD1-768x295.png 768w\" sizes=\"auto, (max-width: 819px) 100vw, 819px\" \/><\/p>\n<p>&nbsp;<\/p>\n<h3><strong>\u767a\u8868\u306e\u30dd\u30a4\u30f3\u30c8<\/strong><\/h3>\n<ul>\n<li>\u5f93\u6765\u306e\u30ce\u30c3\u30af\u30a4\u30f3\u624b\u6cd5\u306f\u52b9\u7387\u306e\u4f4e\u3055\u3068\u3001\u610f\u56f3\u3057\u306a\u3044\u30c9\u30ca\u30fcDNA\u306e\u633f\u5165\u306e\u767a\u751f\u306b\u3088\u308b\u30b2\u30ce\u30e0\u7834\u58ca\u306a\u3069\u306e\u8ab2\u984c\u3092\u62b1\u3048\u3066\u3044\u305f\u3002<\/li>\n<li>DNA\u4fee\u5fa9\u306e\u4ed5\u7d44\u307f\u3092\u4e00\u6642\u7684\u306b\u30d6\u30ed\u30c3\u30af\u3059\u308b\u3053\u3068\u3067\u3001\u90e8\u5206\u7684\u306a\u633f\u5165\u306b\u3088\u308b\u30b2\u30ce\u30e0\u7834\u58ca\u304c\u8d77\u3053\u3089\u306a\u3044\u6b63\u78ba\u306a\u30ce\u30c3\u30af\u30a4\u30f3\u624b\u6cd5\u3092\u78ba\u7acb\u3057\u305f\u3002<\/li>\n<li>\u65b0\u624b\u6cd5\u306b\u3088\u308a90\uff05\u8d85\u306e\u9ad8\u3044\u78ba\u7387\u30672\u7d44\u306e\u67d3\u8272\u4f53\u4e21\u65b9\u306b\u540c\u6642\u306b\u30ce\u30c3\u30af\u30a4\u30f3\u3055\u305b\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u305f\u3002<\/li>\n<\/ul>\n<p>\u65e9\u7a32\u7530\u5927\u5b66\u7406\u5de5\u5b66\u8853\u9662\u306e<a href=\"https:\/\/w-rdb.waseda.jp\/html\/100001237_ja.html\">\u65b0\u4e95\u5927\u7950\uff08\u3042\u3089\u3044\u3060\u3044\u3059\u3051\uff09\u8b1b\u5e2b<\/a>\u304a\u3088\u3073<a href=\"https:\/\/w-rdb.waseda.jp\/html\/100000906_ja.html\">\u4e2d\u5c3e\u6d0b\u4e00\uff08\u306a\u304b\u304a\u3088\u3046\u3044\u3061\uff09\u6559\u6388<\/a>\u3089\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u30b2\u30ce\u30e0\u306e\u72d9\u3063\u305f\u5834\u6240\u306b\u30c9\u30ca\u30fcDNA\u3092\u633f\u5165\u3059\u308b\u3001\u6b63\u78ba\u6027\u3068\u52b9\u7387\u6027\u304c\u9ad8\u3044\u65b0\u305f\u306a\u76f8\u540c\u7d44\u63db\u3048\u30ce\u30c3\u30af\u30a4\u30f3\u624b\u6cd5\u3068\u306a\u308bBiPoD\uff08<u>Bi<\/u>allelic knock-in assisted by <u>Po<\/u>l \u03b8 and <u>D<\/u>NA-PK inhibition\uff09\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002<\/p>\n<p>\u5f93\u6765\u306e\u30ce\u30c3\u30af\u30a4\u30f3\u624b\u6cd5\u306f\u52b9\u7387\u6027\u306e\u4f4e\u3055\u306b\u8ab2\u984c\u3092\u62b1\u3048\u3001\u7814\u7a76\u306e\u8db3\u67b7\u3068\u306a\u3063\u3066\u3044\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u3053\u306e\u624b\u6cd5\u3067\u306f\u3001\u30c9\u30ca\u30fcDNA\u306e\u4e00\u90e8\u5206\u3060\u3051\u304c\u983b\u7e41\u306b\u633f\u5165\u3059\u308b\u305f\u3081\u3001\u3053\u308c\u306b\u3088\u308a\u5143\u3005\u306e\u907a\u4f1d\u5b50\u3092\u7834\u58ca\u3057\u305f\u308a\u691c\u51fa\u304c\u56f0\u96e3\u306b\u306a\u3063\u305f\u308a\u3059\u308b\u3053\u3068\u304c\u5224\u308a\u307e\u3057\u305f\u3002<\/p>\n<p>\u305d\u3053\u3067\u4eca\u56de\u3001\u672c\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u5f93\u6765\u306e\u624b\u6cd5\u3088\u308a\u3082\u76f8\u540c\u7d44\u63db\u3048\u306e\u52b9\u7387\u3092\u9ad8\u3081\u3064\u3064\u3001\u305d\u308c\u4ee5\u5916\u306e\u4ed5\u7d44\u307f\u306b\u3088\u308b\u30c9\u30ca\u30fcDNA\u306e\u633f\u5165\u3092\u307b\u307c\u5b8c\u5168\u306b\u6291\u3048\u3089\u308c\u308b\u65b0\u305f\u306a\u624b\u6cd5\u3092\u78ba\u7acb\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u90e8\u5206\u7684\u633f\u5165\u306a\u3069\u306b\u3088\u308b\u30b2\u30ce\u30e0\u306e\u7834\u58ca\u304c\u8d77\u3053\u3089\u306a\u3044\u6b63\u78ba\u3067\u5b89\u5168\u306a\u30ce\u30c3\u30af\u30a4\u30f3\u304c\u53ef\u80fd\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u307e\u305f\u3053\u306e\u65b0\u624b\u6cd5\u306f90%\u8d85\u306e\u9ad8\u3044\u78ba\u7387\u30672\u7d44\u306e\u67d3\u8272\u4f53\u4e21\u65b9\u306b\u540c\u6642\u306b\u30ce\u30c3\u30af\u30a4\u30f3\u3055\u305b\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u307e\u3057\u305f\u3002\u6b63\u78ba\u6027\u3068\u52b9\u7387\u304c\u9ad8\u307e\u3063\u305f\u65b0\u624b\u6cd5\u306f\u3001\u30de\u30a6\u30b9ES\u7d30\u80de\u3092\u7528\u3044\u305f\u7814\u7a76\u3092\u52a0\u901f\u3055\u305b\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u3055\u3089\u306b\u3001\u7c21\u4fbf\u306a\u624b\u6cd5\u3067\u7279\u5225\u306a\u6a5f\u5668\u3084\u9ad8\u4fa1\u306a\u8a66\u85ac\u3092\u5fc5\u8981\u3068\u3057\u306a\u3044\u305f\u3081\u306b\u3001\u5c0e\u5165\u304c\u5bb9\u6613\u306a\u305f\u3081\u3001\u76f8\u540c\u7d44\u63db\u3048\u30ce\u30c3\u30af\u30a4\u30f3\u624b\u6cd5\u306e\u65b0\u305f\u306a\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3068\u3057\u3066\u5e83\u304f\u666e\u53ca\u3059\u308b\u3053\u3068\u304c\u671f\u5f85\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>\u672c\u7814\u7a76\u6210\u679c\u306f\u3001Springer Nature\u793e\u300e<a href=\"https:\/\/www.nature.com\/srep\/\">Scientific Reports<\/a>\u300f\u306e\u30aa\u30f3\u30e9\u30a4\u30f3\u7248\u306b2021\u5e749\u670813\u65e5\uff08\u73fe\u5730\u6642\u9593\uff09\u306b\u63b2\u8f09\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n<h3>\u3010\u8ad6\u6587\u60c5\u5831\u3011<\/h3>\n<p>\u96d1\u8a8c\u540d\uff1a<a href=\"https:\/\/www.nature.com\/srep\/\">Scientific Reports<\/a><br \/>\n\u8ad6\u6587\u540d\uff1a<a href=\"http:\/\/www.nature.com\/articles\/s41598-021-97579-8\">Efficient biallelic knock-in in mouse embryonic stem cells by in vivo-linearization of donor and transient inhibition of DNA polymerase \u03b8\/DNA-PK<\/a><br \/>\nDOI\uff1a<a href=\"http:\/\/10.1038\/s41598-021-97579-8\">10.1038\/s41598-021-97579-8<\/a><\/p>\n<h3>\uff11\uff0e\u3053\u308c\u307e\u3067\u306e\u7814\u7a76\u3067\u5206\u304b\u3063\u3066\u3044\u305f\u3053\u3068\uff08\u79d1\u5b66\u53f2\u7684\u30fb\u6b74\u53f2\u7684\u306a\u80cc\u666f\u306a\u3069\uff09<\/h3>\n<p>\u751f\u547d\u306e\u8a2d\u8a08\u56f3\u3067\u3042\u308b\u30b2\u30ce\u30e0DNA\u306e\u4e2d\u306b\u3042\u308b\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u3092\u7834\u58ca\u3057\u305f\u308a\u3001\u5916\u6765\u306e\u30c9\u30ca\u30fcDNA<sup>\u203b1<\/sup>\u3092\u72d9\u3063\u305f\u5834\u6240\u306b\u633f\u5165\uff08\u30ce\u30c3\u30af\u30a4\u30f3\uff09\u3057\u305f\u308a\u3059\u308b\u6280\u8853\u306f\u57fa\u790e\u7814\u7a76\u306b\u6b20\u304b\u305b\u306a\u3044\u3082\u306e\u3067\u3059\u3002\u307e\u305f\u3001\u907a\u4f1d\u5b50\u6cbb\u7642\u3084\u7d30\u80de\u533b\u7642\u3068\u3044\u3063\u305f\u6700\u5148\u7aef\u533b\u7642\u3078\u306e\u6d3b\u7528\u3082\u671f\u5f85\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>CRISPR\/Cas9\u30b7\u30b9\u30c6\u30e0<sup>\u203b2<\/sup>\u3092\u7d30\u80de\u304c\u5143\u3005\u6301\u3063\u3066\u3044\u308bDNA\u4fee\u5fa9\u306e\u4ed5\u7d44\u307f\u3067\u3042\u308b\u76f8\u540c\u7d44\u63db\u3048<sup>\u203b3<\/sup>\u306b\u7d44\u307f\u5408\u308f\u305b\u305f\u30ce\u30c3\u30af\u30a4\u30f3\u306f\u3001\u4e3b\u8981\u306a\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u3068\u3057\u3066\u73fe\u5728\u3082\u4e16\u754c\u4e2d\u3067\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u4eba\u9593\u306e\u7d30\u80de\u306e\u4e2d\u306b\u306f\u907a\u4f1d\u5b50\u306e\u30bb\u30c3\u30c8\u3067\u3042\u308b\u67d3\u8272\u4f53\u304c2\u7d44\u5b58\u5728\u3057\u3066\u304a\u308a\u3001\u540c\u3058\u907a\u4f1d\u5b50\u304c\uff12\u3064\u305a\u3064\u5b58\u5728\u3057\u3066\u3044\u307e\u3059\u3002\u3088\u3063\u3066\u30ce\u30c3\u30af\u30a4\u30f3\u306b\u3088\u308a\u907a\u4f1d\u5b50\u306e\u50cd\u304d\u3092\u64cd\u4f5c\u3059\u308b\u5834\u5408\u3001\u30c9\u30ca\u30fcDNA\u3092\u4e21\u65b9\u306e\u67d3\u8272\u4f53\u306b\u633f\u5165\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u76f8\u540c\u7d44\u63db\u3048\u306b\u3088\u308a\uff12\u7d44\u306e\u67d3\u8272\u4f53\u306b\u540c\u6642\u306b\u30ce\u30c3\u30af\u30a4\u30f3\u3055\u308c\u308b\u78ba\u7387\u306f\u6570%\u672a\u6e80\u3068\u975e\u5e38\u306b\u4f4e\u304f\u3001\u7814\u7a76\u3092\u9032\u3081\u308b\u4e0a\u3067\u306e\u8db3\u67b7\u3068\u306a\u3063\u3066\u3044\u307e\u3057\u305f\u3002<\/p>\n<p>\u307e\u305f\u5f93\u6765\u306e\u624b\u6cd5\u3067\u306f\u3001\u76f8\u540c\u7d44\u63db\u3048\u30ce\u30c3\u30af\u30a4\u30f3\u3068\u540c\u6642\u306b\u3001\u6a19\u7684\u3068\u7121\u95a2\u4fc2\u306a\u5834\u6240\u3078\u306e\u30c9\u30ca\u30fcDNA\u306e\u30e9\u30f3\u30c0\u30e0\u306a\u633f\u5165\u304c\u767a\u751f\u3057\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u30e9\u30f3\u30c0\u30e0\u306a\u633f\u5165\u306b\u3088\u308a\u633f\u5165\u7b87\u6240\u306b\u5143\u3005\u3042\u3063\u305f\u907a\u4f1d\u5b50\u306a\u3069\u304c\u7834\u58ca\u3055\u308c\u308b\u3068\u3001\u7d30\u80de\u306b\u4e88\u671f\u305b\u306c\u5f71\u97ff\u3092\u53ca\u307c\u3057\u307e\u3059\u3002\u3053\u308c\u306f\u30ce\u30c3\u30af\u30a4\u30f3\u3092\u81e8\u5e8a\u306b\u5fdc\u7528\u3059\u308b\u306b\u3042\u305f\u308a\u5fc5\u305a\u89e3\u6c7a\u3057\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\u554f\u984c\u3067\u3057\u305f\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-74934 size-full\" src=\"https:\/\/www.waseda.jp\/top\/assets\/uploads\/2021\/09\/BiPoD2.png\" alt=\"\" width=\"501\" height=\"236\" \/><\/p>\n<h3>\uff12\uff0e\u4eca\u56de\u306e\u7814\u7a76\u3067\u65b0\u305f\u306b\u5b9f\u73fe\u3057\u3088\u3046\u3068\u3057\u305f\u3053\u3068\u3001\u660e\u3089\u304b\u306b\u306a\u3063\u305f\u3053\u3068<\/h3>\n<p>\u672c\u7814\u7a76\u3067\u306f\u3001\u30de\u30a6\u30b9\u306eES\u7d30\u80de\u3092\u5bfe\u8c61\u306b\u3001\u6b63\u78ba\u6027\u3068\u52b9\u7387\u3092\u9ad8\u3081\u305f\u65b0\u305f\u306a\u76f8\u540c\u7d44\u63db\u3048\u30ce\u30c3\u30af\u30a4\u30f3\u624b\u6cd5\u306e\u78ba\u7acb\u306b\u53d6\u308a\u7d44\u307f\u307e\u3057\u305f\u3002<\/p>\n<p>\u3053\u308c\u307e\u3067\u306f\u76f8\u540c\u7d44\u63db\u3048\u30ce\u30c3\u30af\u30a4\u30f3\u3092\u884c\u3046\u5834\u5408\u3001\u30c9\u30ca\u30fcDNA\u306b\u8907\u6570\u306e\u30de\u30fc\u30ab\u30fc\u907a\u4f1d\u5b50<sup>\u203b4<\/sup>\u3092\u914d\u7f6e\u3059\u308b\u3053\u3068\u3067\u3001\u6b63\u3057\u304f\u30ce\u30c3\u30af\u30a4\u30f3\u3055\u308c\u305f\u7d30\u80de\u3068\u3001\u76f8\u540c\u7d44\u63db\u3048\u4ee5\u5916\u306e\u4ed5\u7d44\u307f\u306b\u3088\u308a\u30c9\u30ca\u30fcDNA\u5168\u4f53\u304c\u30e9\u30f3\u30c0\u30e0\u306b\u633f\u5165\u3055\u308c\u305f\u7d30\u80de\u3092\u533a\u5225\u3057\u3001\u5f8c\u8005\u306e\u7d30\u80de\u3092\u9664\u5916\u3059\u308b\u3068\u3044\u3046\u6226\u7565\u304c\u3068\u3089\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u672c\u7814\u7a76\u3092\u9032\u3081\u308b\u904e\u7a0b\u3067\u3001\u901a\u5e38\u306e\u624b\u6cd5\u3067\u306f\u30c9\u30ca\u30fcDNA\u306e\u4e00\u90e8\u5206\u3060\u3051\u306e\u30e9\u30f3\u30c0\u30e0\u306a\u633f\u5165\u304c\u983b\u7e41\u306b\u767a\u751f\u3059\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002\u3053\u308c\u306f\u633f\u5165\u7b87\u6240\u306b\u5143\u3005\u3042\u308b\u907a\u4f1d\u5b50\u3092\u7834\u58ca\u3057\u3066\u3057\u307e\u3046\u4e0a\u306b\u3001\u633f\u5165\u306e\u4ed5\u65b9\u306b\u3088\u3063\u3066\u306f\u30de\u30fc\u30ab\u30fc\u907a\u4f1d\u5b50\u306b\u3088\u308b\u691c\u51fa\u304c\u4e0d\u53ef\u80fd\u306b\u306a\u308a\u307e\u3059\u3002\u3088\u3063\u3066\u5f8c\u304b\u3089\u30de\u30fc\u30ab\u30fc\u907a\u4f1d\u5b50\u306b\u3088\u3063\u3066\u9664\u5916\u3059\u308b\u306e\u3067\u306f\u306a\u304f\u3001\u6700\u521d\u304b\u3089\u90e8\u5206\u7684\u633f\u5165\u304c\u8d77\u3053\u3089\u306a\u3044\u3088\u3046\u306a\u624b\u6cd5\u304c\u5fc5\u8981\u3068\u8003\u3048\u3089\u308c\u307e\u3057\u305f\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-74936\" src=\"https:\/\/www.waseda.jp\/top\/assets\/uploads\/2021\/09\/BiPoD3.png\" alt=\"\" width=\"722\" height=\"210\" srcset=\"https:\/\/www.waseda.jp\/top\/assets\/uploads\/2021\/09\/BiPoD3.png 722w, https:\/\/www.waseda.jp\/top\/assets\/uploads\/2021\/09\/BiPoD3-610x177.png 610w, https:\/\/www.waseda.jp\/top\/assets\/uploads\/2021\/09\/BiPoD3-720x210.png 720w\" sizes=\"auto, (max-width: 722px) 100vw, 722px\" \/><\/p>\n<p>\u90e8\u5206\u7684\u633f\u5165\u306f\u76f8\u540c\u7d44\u63db\u3048\u4ee5\u5916\u306eDNA\u4fee\u5fa9\u306e\u4ed5\u7d44\u307f\uff08\u975e\u76f8\u540c\u672b\u7aef\u7d50\u5408\u3001\u30de\u30a4\u30af\u30ed\u30db\u30e2\u30ed\u30b8\u30fc\u5a92\u4ecb\u672b\u7aef\u7d50\u5408\uff09\u3092\u4ecb\u3057\u3066\u8d77\u3053\u308a\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u4ed5\u7d44\u307f\u3092\u4e00\u6642\u7684\u306b\u30d6\u30ed\u30c3\u30af\u3059\u308b\u3053\u3068\u3067\u7279\u7570\u7684\u306a\u76f8\u540c\u7d44\u63db\u3048\u30ce\u30c3\u30af\u30a4\u30f3\u304c\u9054\u6210\u3067\u304d\u306a\u3044\u304b\u691c\u8a0e\u3057\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001\u30c9\u30ca\u30fcDNA\u306e\u69cb\u9020\u306e\u5de5\u592b\u3001\u5c0f\u5206\u5b50\u5316\u5408\u7269M3814<sup>\u203b5<\/sup>\u306b\u3088\u308b\u975e\u76f8\u540c\u672b\u7aef\u7d50\u5408\u306e\u963b\u5bb3\u3001<em>Polq<\/em>\u907a\u4f1d\u5b50<sup>\u203b6<\/sup>\u306b\u5bfe\u3059\u308bshRNA<sup>\u203b7<\/sup>\u306b\u3088\u308b\u30de\u30a4\u30af\u30ed\u30db\u30e2\u30ed\u30b8\u30fc\u5a92\u4ecb\u672b\u7aef\u7d50\u5408\u306e\u963b\u5bb3\u3092\u7d44\u307f\u5408\u308f\u305b\u308b\u3053\u3068\u3067\u3001\u901a\u5e38\u6cd5\u3088\u308a\u3082\u76f8\u540c\u7d44\u63db\u3048\u306e\u52b9\u7387\u3092\u9ad8\u3081\u3064\u3064\u3001\u305d\u308c\u4ee5\u5916\u306e\u4ed5\u7d44\u307f\u306b\u3088\u308b\u30c9\u30ca\u30fcDNA\u306e\u633f\u5165\u3092\u307b\u307c\u5b8c\u5168\u306b\u6291\u3048\u3089\u308c\u308b\u624b\u6cd5\u3092\u78ba\u7acb\u3057\u307e\u3057\u305f\u3002<\/p>\n<p>\u672c\u624b\u6cd5\u306b\u3088\u3063\u3066\u3001\u90e8\u5206\u7684\u633f\u5165\u306a\u3069\u306b\u3088\u308b\u30b2\u30ce\u30e0\u306e\u7834\u58ca\u304c\u8d77\u3053\u3089\u306a\u3044\u6b63\u78ba\u306a\u30ce\u30c3\u30af\u30a4\u30f3\u304c\u53ef\u80fd\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u3053\u308c\u306b\u3088\u308a\u7d30\u80de\u3078\u306e\u4e88\u671f\u305b\u306c\u60aa\u5f71\u97ff\u3092\u9632\u3050\u3053\u3068\u304c\u3067\u304d\u3001\u907a\u4f1d\u5b50\u6cbb\u7642\u3084\u7d30\u80de\u533b\u7642\u306b\u30ce\u30c3\u30af\u30a4\u30f3\u3092\u7528\u3044\u308b\u969b\u306e\u5b89\u5168\u6027\u304c\u5927\u5e45\u306b\u5411\u4e0a\u3057\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u4e88\u60f3\u5916\u306e\u767a\u898b\u3068\u3057\u3066\u3001\u3053\u306e\u624b\u6cd5\u3067\u306f90\uff05\u8d85\u306e\u78ba\u7387\u3067\uff12\u7d44\u306e\u67d3\u8272\u4f53\u4e21\u65b9\u306b\u540c\u6642\u306b\u30ce\u30c3\u30af\u30a4\u30f3\u304c\u8d77\u3053\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\uff08\u901a\u5e38\u6cd5\u3067\u306f6%\uff09\u3002\u76f8\u540c\u7d44\u63db\u3048\u30ce\u30c3\u30af\u30a4\u30f3\u306e\u6539\u826f\u6cd5\u306b\u3064\u3044\u3066\u306f\u3053\u308c\u307e\u3067\u306b\u591a\u6570\u306e\u5831\u544a\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u305d\u308c\u3089\u3068\u6bd4\u3079\u3066\u3082\u4e21\u67d3\u8272\u4f53\u3078\u306e\u540c\u6642\u30ce\u30c3\u30af\u30a4\u30f3\u52b9\u7387\u306f\u9065\u304b\u306b\u9ad8\u3044\u3082\u306e\u3067\u3057\u305f\u3002\u305d\u3053\u3067\u672c\u624b\u6cd5\u3092\u65b0\u305f\u306a\u76f8\u540c\u7d44\u63db\u3048\u30ce\u30c3\u30af\u30a4\u30f3\u624b\u6cd5\u3068\u3057\u3066BiPoD\uff08<u>Bi<\/u>allelic knock-in assisted by <u>Po<\/u>l \u03b8 and <u>D<\/u>NA-PK inhibition\uff09\u3068\u547d\u540d\u3057\u307e\u3057\u305f\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-74937\" src=\"https:\/\/www.waseda.jp\/top\/assets\/uploads\/2021\/09\/BiPoD4.png\" alt=\"\" width=\"686\" height=\"417\" srcset=\"https:\/\/www.waseda.jp\/top\/assets\/uploads\/2021\/09\/BiPoD4.png 686w, https:\/\/www.waseda.jp\/top\/assets\/uploads\/2021\/09\/BiPoD4-610x371.png 610w\" sizes=\"auto, (max-width: 686px) 100vw, 686px\" \/><\/p>\n<h6><strong>\u56f3\u5de6\u4e0a<\/strong>\uff1aBiPoD\u306e\u6982\u7565\u56f3\u3002<\/h6>\n<h6><strong>\u56f3\u53f3\u4e0a<\/strong>\uff1a\u30e9\u30f3\u30c0\u30e0\u306a\u633f\u5165\u306e\u767a\u751f\u7387\u3002M3814\u3068<em>Polq<\/em> shRNA\u3092\u7d44\u307f\u5408\u308f\u305b\u308b\u3053\u3068\u3067\u30e9\u30f3\u30c0\u30e0\u633f\u5165\u304c\u5927\u5e45\u306b\u6291\u3048\u3089\u308c\u3066\u3044\u308b\u3002<\/h6>\n<h6><strong>\u56f3\u4e0b<\/strong>\uff1a\u30b2\u30ce\u30e0PCR\u6cd5\u306b\u3088\u308b\u30ce\u30c3\u30af\u30a4\u30f3\u72b6\u6cc1\u306e\u78ba\u8a8d\u3002\u901a\u5e38\u6cd516\u7a2e\uff08\u4e0a\u6bb5\uff09\u3001BiPoD\u6761\u4ef621\u7a2e\uff08\u4e0b\u6bb5\uff09\u306e\u30af\u30ed\u30fc\u30f3\u3092\u5358\u96e2\u3001\u89e3\u6790\u3057\u3066\u3044\u308b\u3002\u5404\u30af\u30ed\u30fc\u30f3\u306b\u3064\u3044\u3066\u3001\u4e0a\u5074\u306e\u30d0\u30f3\u30c9\u304c\u30ce\u30c3\u30af\u30a4\u30f3\u3055\u308c\u305f\u67d3\u8272\u4f53\u3001\u4e0b\u5074\u306e\u30d0\u30f3\u30c9\u304c\u30ce\u30c3\u30af\u30a4\u30f3\u3055\u308c\u306a\u304b\u3063\u305f\u67d3\u8272\u4f53\u3092\u793a\u3057\u3066\u304a\u308a\u3001\u4e0a\u5074\u3060\u3051\u306b\u30d0\u30f3\u30c9\u304c\u3042\u308b\u30af\u30ed\u30fc\u30f3\u306f\u4e21\u67d3\u8272\u4f53\u306b\u30ce\u30c3\u30af\u30a4\u30f3\u304c\u8d77\u3053\u3063\u3066\u3044\u308b\u3002BiPoD\u306b\u3088\u308a21\u30af\u30ed\u30fc\u30f3\u4e2d20\u30af\u30ed\u30fc\u30f3\uff0895%\uff09\u304c\u4e21\u67d3\u8272\u4f53\u30ce\u30c3\u30af\u30a4\u30f3\u3068\u306a\u308a\u3001\u901a\u5e38\u6cd5\uff0816\u30af\u30ed\u30fc\u30f3\u4e2d1\u30af\u30ed\u30fc\u30f3\u30016%\uff09\u3088\u308a\u3082\u4e21\u67d3\u8272\u4f53\u30ce\u30c3\u30af\u30a4\u30f3\u7387\u304c\u5927\u5e45\u306b\u5411\u4e0a\u3057\u305f\u3002<\/h6>\n<p>\u307e\u305fBiPoD\u3092\u7528\u3044\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u901a\u5e38\u6cd5\u3067\u306f\u4e0d\u53ef\u80fd\u3060\u3063\u305f\uff12\u7b87\u6240\u306e\u6a19\u7684\u3078\u306e\u4e21\u67d3\u8272\u4f53\u540c\u6642\uff08\u8a08\uff14\u7b87\u6240\uff09\u30ce\u30c3\u30af\u30a4\u30f3\u3082\u9ad8\u52b9\u7387\u306b\u9054\u6210\u3067\u304d\u308b\u3053\u3068\u3082\u793a\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-74935\" src=\"https:\/\/www.waseda.jp\/top\/assets\/uploads\/2021\/09\/BiPoD5.png\" alt=\"\" width=\"662\" height=\"216\" srcset=\"https:\/\/www.waseda.jp\/top\/assets\/uploads\/2021\/09\/BiPoD5.png 662w, https:\/\/www.waseda.jp\/top\/assets\/uploads\/2021\/09\/BiPoD5-610x199.png 610w\" sizes=\"auto, (max-width: 662px) 100vw, 662px\" \/><\/p>\n<h6><strong>\u56f3<\/strong>\uff1a\uff12\u90e8\u4f4d\uff08<em>Rosa26a<\/em>\u3001<em>Sox2<\/em>\uff09\u3078\u306e\u540c\u6642\u30ce\u30c3\u30af\u30a4\u30f3\u3092\u884c\u3044\u3001\u30b2\u30ce\u30e0PCR\u6cd5\u306b\u3088\u308a\u30af\u30ed\u30fc\u30f3\u306e\u89e3\u6790\u3092\u884c\u306a\u3063\u305f\u3002\u5404\u90e8\u4f4d\u306b\u3064\u3044\u3066\u3001\u4e21\u67d3\u8272\u4f53\uff08Bi-KI\uff09\u3001\u7247\u65b9\u306e\u67d3\u8272\u4f53\uff08Mono-KI\uff09\u3001\u30ce\u30c3\u30af\u30a4\u30f3\u306a\u3057\uff08Other\uff09\u306e\u3044\u305a\u308c\u3067\u3042\u308b\u304b\u3092\u89e3\u6790\u3057\u3001\u30af\u30ed\u30fc\u30f3\u6570\u3068\u30d1\u30fc\u30bb\u30f3\u30c6\u30fc\u30b8\u3092\u793a\u3057\u305f\u3002\u901a\u5e38\u6cd5\u3067\u306f\u4e21\u67d3\u8272\u4f53\u30ce\u30c3\u30af\u30a4\u30f3\u306f\u3069\u3061\u3089\u306e\u90e8\u4f4d\u306b\u3082\u8d77\u3053\u3063\u3066\u3044\u306a\u304b\u3063\u305f\u304c\u3001BiPoD\u3067\u306f9\u30af\u30ed\u30fc\u30f3\uff0839%\uff09\u304c<em>Rosa26a<\/em>\u306b\u4e21\u67d3\u8272\u4f53\u30ce\u30c3\u30af\u30a4\u30f3\u3055\u308c\u3066\u304a\u308a\u3001\u3046\u30616\u30af\u30ed\u30fc\u30f3\uff0826%\uff09\u306f<em>Sox2<\/em>\u306b\u3082\u4e21\u67d3\u8272\u4f53\u30ce\u30c3\u30af\u30a4\u30f3\u304c\u78ba\u8a8d\u3055\u308c\u30014\u7b87\u6240\u540c\u6642\u306b\u30ce\u30c3\u30af\u30a4\u30f3\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u305f\u3002<\/h6>\n<h3>\uff13\uff0e\u7814\u7a76\u306e\u6ce2\u53ca\u52b9\u679c\u3084\u793e\u4f1a\u7684\u5f71\u97ff<\/h3>\n<p>\u9ad8\u52b9\u7387\u304b\u3064\u7279\u7570\u6027\u306e\u9ad8\u3044\u624b\u6cd5\u3067\u3042\u308bBiPoD\u306f\u30de\u30fc\u30ab\u30fc\u907a\u4f1d\u5b50\u3092\u4f7f\u308f\u305a\u306b\u4e21\u67d3\u8272\u4f53\u30ce\u30c3\u30af\u30a4\u30f3\u7d30\u80de\u3092\u6a39\u7acb\u3059\u308b\u3053\u3068\u3084\u3001\u30ef\u30f3\u30b9\u30c6\u30c3\u30d7\u3067\u8907\u6570\u306e\u30ce\u30c3\u30af\u30a4\u30f3\u3092\u65bd\u3059\u3053\u3068\u3092\u53ef\u80fd\u3068\u3057\u3001\u30de\u30a6\u30b9ES\u7d30\u80de\u3092\u7528\u3044\u305f\u7814\u7a76\u3092\u5927\u5e45\u306b\u52a0\u901f\u3055\u305b\u308b\u30dd\u30c6\u30f3\u30b7\u30e3\u30eb\u3092\u6709\u3057\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u975e\u5e38\u306b\u7c21\u4fbf\u306a\u624b\u6cd5\u3067\u7279\u5225\u306a\u6a5f\u5668\u3084\u9ad8\u4fa1\u306a\u8a66\u85ac\u3082\u5fc5\u8981\u3068\u3057\u306a\u3044\u306e\u3067\u3001\u7814\u7a76\u5ba4\u3078\u306e\u5c0e\u5165\u3082\u5bb9\u6613\u3067\u3059\u3002\u76f8\u540c\u7d44\u63db\u3048\u30ce\u30c3\u30af\u30a4\u30f3\u624b\u6cd5\u306e\u65b0\u305f\u306a\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3068\u3057\u3066\u5e83\u304f\u666e\u53ca\u3057\u3066\u3044\u304f\u3053\u3068\u3092\u671f\u5f85\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<h3>\uff14\uff0e\u4eca\u5f8c\u306e\u8ab2\u984c<\/h3>\n<p>BiPoD\u304c\u30de\u30a6\u30b9ES\u7d30\u80de\u4ee5\u5916\u306e\u7d30\u80de\u3001\u7279\u306b\u30d2\u30c8\u306e\u7d30\u80de\u306b\u5bfe\u3057\u3066\u3082\u6709\u52b9\u304b\u3069\u3046\u304b\u3092\u78ba\u8a8d\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u307e\u307e\u306e\u624b\u6cd5\u3067\u5341\u5206\u306a\u52b9\u679c\u304c\u898b\u3089\u308c\u306a\u3044\u5834\u5408\u3001\u4f55\u3089\u304b\u306e\u5fae\u4fee\u6b63\u3092\u52a0\u3048\u3066\u3044\u304f\u5fc5\u8981\u304c\u3042\u308b\u3067\u3057\u3087\u3046\u3002\u307e\u305f\u3001shRNA\u306b\u3088\u308b\u907a\u4f1d\u5b50\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u3092\u4f55\u3089\u304b\u306e\u85ac\u5264\u306b\u7f6e\u304d\u63db\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u308c\u3070\u3001\u4f5c\u696d\u304c\u3055\u3089\u306b\u7c21\u6613\u306a\u3082\u306e\u306b\u306a\u308a\u307e\u3059\u3002\u30b7\u30f3\u30d7\u30eb\u306a\u624b\u6cd5\u306a\u3060\u3051\u306b\u3001\u6539\u826f\u306e\u4f59\u5730\u306f\u307e\u3060\u307e\u3060\u6b8b\u3055\u308c\u3066\u3044\u308b\u3068\u8003\u3048\u3066\u3044\u307e\u3059\u3002\u4e21\u67d3\u8272\u4f53\u3078\u306e\u30ce\u30c3\u30af\u30a4\u30f3\u52b9\u7387\u304c\u5287\u7684\u306b\u4e0a\u6607\u3059\u308b\u30e1\u30ab\u30cb\u30ba\u30e0\u306e\u8a73\u7d30\u306b\u3064\u3044\u3066\u3082\u3001\u4eca\u5f8c\u8abf\u3079\u3066\u3044\u304f\u4e88\u5b9a\u3067\u3059\u3002<\/p>\n<h3>\uff15\uff0e\u7814\u7a76\u8005\u306e\u30b3\u30e1\u30f3\u30c8<\/h3>\n<p>\u5143\u3005\u306f\u81ea\u8eab\u306e\u65e5\u3005\u306e\u5b9f\u9a13\u3092\u52b9\u7387\u5316\u3059\u308b\u305f\u3081\u306b\u59cb\u3081\u305f\u65e5\u66dc\u5927\u5de5\u306e\u3088\u3046\u306a\u30c6\u30fc\u30de\u3067\u3057\u305f\u304c\u3001\u4e88\u60f3\u3092\u4e0a\u56de\u308b\u512a\u308c\u305f\u52b9\u679c\u304c\u8a8d\u3081\u3089\u308c\u305f\u305f\u3081\u3001\u4e16\u306e\u4e2d\u306b\u5e83\u304f\u77e5\u3089\u308c\u3066\u307b\u3057\u3044\u3068\u8003\u3048\u3001\u8ad6\u6587\u306b\u307e\u3068\u3081\u3066\u767a\u8868\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u624b\u6cd5\u304c\u81ea\u5206\u306e\u7814\u7a76\u3060\u3051\u3067\u306a\u304f\u3001\u56fd\u5185\u5916\u306e\u591a\u304f\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306b\u6d3b\u7528\u3055\u308c\u3001\u65b0\u305f\u306a\u79d1\u5b66\u7684\u767a\u898b\u306e\u4e00\u52a9\u3068\u306a\u308b\u3053\u3068\u3092\u9858\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n<h3>\uff16\uff0e\u7528\u8a9e\u89e3\u8aac<\/h3>\n<h4>\u203b1\u3000\u30c9\u30ca\u30fcDNA<\/h4>\n<ul>\n<li>\u7d30\u80de\u5916\u304b\u3089\u30b2\u30ce\u30e0\u306b\u633f\u5165\u3055\u308c\u308bDNA\u914d\u5217\u3002\u5916\u6765\u907a\u4f1d\u5b50\u306e\u633f\u5165\u3084\u5143\u3005\u3042\u308b\u907a\u4f1d\u5b50\u306b\u4eba\u5de5\u7684\u306a\u914d\u5217\u3092\u4ed8\u52a0\u3059\u308b\u306a\u3069\u3001\u76ee\u7684\u306b\u5fdc\u3058\u3066\u81ea\u7531\u306b\u8a2d\u8a08\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3002<\/li>\n<\/ul>\n<h4>\u203b2\u3000CRISPR\/Cas9\u30b7\u30b9\u30c6\u30e0<\/h4>\n<ul>\n<li>2012\u5e74\u306b\u958b\u767a\u3055\u308c\u305f\u3001\u30ac\u30a4\u30c9RNA\u3068\u547c\u3070\u308c\u308bRNA\u3068Cas9\u3068\u3044\u3046\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7d30\u80de\u306b\u5c0e\u5165\u3059\u308b\u3053\u3068\u3067\u3001\u30b2\u30ce\u30e0DNA\u306e\u72d9\u3063\u305f\u5834\u6240\u3060\u3051\u3092\u7279\u7570\u7684\u306b\u5207\u65ad\u3059\u308b\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u3002<\/li>\n<\/ul>\n<h4>\u203b3\u3000\u76f8\u540c\u7d44\u63db\u3048<\/h4>\n<ul>\n<li>\u5207\u65ad\u3055\u308c\u305f\u30b2\u30ce\u30e0DNA\u3092\u4fee\u5fa9\u3059\u308b\u4ed5\u7d44\u307f\u306e\u4e00\u3064\u3067\u3001\u5207\u65ad\u7b87\u6240\u306e\u4e21\u5074\u3068\u540c\u3058\u914d\u5217\uff08\u30a2\u30fc\u30e0\uff09\u3092\u6301\u3064DNA\u3092\u92f3\u578b\u3068\u3057\u3066\u4fee\u5fa9\u3092\u884c\u3046\u3002\u4fee\u5fa9\u306e\u92f3\u578b\u3068\u3057\u3066\u30c9\u30ca\u30fcDNA\u304c\u4f7f\u308f\u308c\u308b\u3068\u3001\u30a2\u30fc\u30e0\u306b\u631f\u307e\u308c\u305fDNA\u914d\u5217\u3082\u4e00\u7dd2\u306b\u633f\u5165\u3055\u308c\u308b\u3002CRISPR\/Cas9\u30b7\u30b9\u30c6\u30e0\u306b\u3088\u308a\u30b2\u30ce\u30e0DNA\u3092\u5207\u65ad\u3059\u308b\u3053\u3068\u3067\u3001\u72d9\u3063\u305f\u5834\u6240\u306b\u52b9\u7387\u7684\u306b\u76f8\u540c\u7d44\u63db\u3048\u3092\u5f15\u304d\u8d77\u3053\u3059\u3053\u3068\u304c\u3067\u304d\u308b\u3002<\/li>\n<\/ul>\n<h4>\u203b4\u3000\u30de\u30fc\u30ab\u30fc\u907a\u4f1d\u5b50<\/h4>\n<ul>\n<li>\u30c9\u30ca\u30fcDNA\u304c\u30b2\u30ce\u30e0\u306b\u633f\u5165\u3055\u308c\u305f\u3053\u3068\u3092\u793a\u3059\u76ee\u5370\u3068\u306a\u308b\u907a\u4f1d\u5b50\u3002\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3084\u6297\u751f\u7269\u8cea\u306b\u5bfe\u3059\u308b\u8010\u6027\u7269\u8cea\u3092\u4f5c\u308b\u907a\u4f1d\u5b50\u304c\u7528\u3044\u3089\u308c\u308b\u3002\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc\u3084\u6297\u751f\u7269\u8cea\u51e6\u7406\u306b\u3088\u308a\u30de\u30fc\u30ab\u30fc\u907a\u4f1d\u5b50\u3092\u6301\u3064\u7d30\u80de\u3060\u3051\u3092\u96c6\u3081\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3002<\/li>\n<\/ul>\n<h4>\u203b5\u3000M3814<\/h4>\n<ul>\n<li>DNA-PK\u3068\u3044\u3046\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u50cd\u304d\u3092\u963b\u5bb3\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u975e\u76f8\u540c\u672b\u7aef\u7d50\u5408\u3092\u30d6\u30ed\u30c3\u30af\u3059\u308b\u85ac\u5264\u3002<\/li>\n<\/ul>\n<h4>\u203b6\u3000<em>Polq<\/em>\u907a\u4f1d\u5b50<\/h4>\n<ul>\n<li>\u30de\u30a4\u30af\u30ed\u30db\u30e2\u30ed\u30b8\u30fc\u5a92\u4ecb\u672b\u7aef\u7d50\u5408\u306b\u5fc5\u9808\u306e\u56e0\u5b50\u3067\u3042\u308bDNA polymerase \u03b8\uff08Pol \u03b8\uff09\u3092\u30b3\u30fc\u30c9\u3057\u3066\u3044\u308b\u907a\u4f1d\u5b50\u3002<\/li>\n<\/ul>\n<h4>\u203b7\u3000shRNA<\/h4>\n<ul>\n<li>\u7d30\u80de\u306b\u5c0e\u5165\u3057\u305f\u30d7\u30e9\u30b9\u30df\u30c9DNA\u304b\u3089\u8ee2\u5199\u3055\u308c\u3001\u30d8\u30a2\u30d4\u30f3\u578b\u306e\u69cb\u9020\u3092\u3068\u3063\u305f\u77ed\u3044RNA\u306e\u3053\u3068\u3067\u3001RNA\u5e72\u6e09\u306b\u3088\u308a\u6a19\u7684\u907a\u4f1d\u5b50\u306e\u50cd\u304d\u3092\u4e00\u6642\u7684\u306b\u6291\u5236\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3002<\/li>\n<\/ul>\n<h3>\uff17\uff0e\u8ad6\u6587\u60c5\u5831<\/h3>\n<p><strong>\u96d1\u8a8c\u540d\uff1a<\/strong><a href=\"https:\/\/www.nature.com\/srep\/\">Scientific Reports<\/a><br \/>\n<strong>\u8ad6\u6587\u540d\uff1a<\/strong>Efficient biallelic knock-in in mouse embryonic stem cells by in vivo-linearization of donor and transient inhibition of DNA polymerase \u03b8\/DNA-PK<br \/>\n<strong>\u57f7\u7b46\u8005\u540d\uff08\u6240\u5c5e\u6a5f\u95a2\u540d\uff09\uff1a<\/strong><a href=\"https:\/\/w-rdb.waseda.jp\/html\/100001237_ja.html\">\u65b0\u4e95\u5927\u7950<\/a><sup>1*<\/sup>\u3001<a href=\"https:\/\/w-rdb.waseda.jp\/html\/100000906_ja.html\">\u4e2d\u5c3e\u6d0b\u4e00<\/a><sup>1,2<\/sup>\uff081\uff1a\u65e9\u7a32\u7530\u5927\u5b66 \u5148\u9032\u7406\u5de5\u5b66\u90e8 \u5316\u5b66\u30fb\u751f\u547d\u5316\u5b66\u79d1\u30012\uff1a\u65e9\u7a32\u7530\u5927\u5b66 \u7406\u5de5\u5b66\u8853\u9662\u7dcf\u5408\u7814\u7a76\u6240\u3001*\u8cac\u4efb\u8457\u8005\uff09<br \/>\n<strong>\u63b2\u8f09\u65e5\u6642\uff08\u73fe\u5730\u6642\u9593\uff09\uff1a<\/strong>2021\u5e749\u670813\u65e510:00<br \/>\n<strong>\u63b2\u8f09\u65e5\u6642\uff08\u65e5\u672c\u6642\u9593\uff09\uff1a<\/strong>2021\u5e749\u670813\u65e519:00<br \/>\n<strong>\u63b2\u8f09URL\uff1a<\/strong><a href=\"http:\/\/www.nature.com\/articles\/s41598-021-97579-8\">www.nature.com\/articles\/s41598-021-97579-8<\/a><br \/>\n<strong>DOI\uff1a<\/strong><a href=\"http:\/\/10.1038\/s41598-021-97579-8\">10.1038\/s41598-021-97579-8<\/a><\/p>\n<h3>\uff18\uff0e\u7814\u7a76\u52a9\u6210\uff08\u5916\u90e8\u8cc7\u91d1\u306b\u3088\u308b\u52a9\u6210\u3092\u53d7\u3051\u305f\u7814\u7a76\u5b9f\u65bd\u306e\u5834\u5408\uff09<\/h3>\n<p>\u672c\u7814\u7a76\u306f\u65e5\u672c\u5b66\u8853\u632f\u8208\u4f1a\uff08JSPS\uff09\u79d1\u5b66\u7814\u7a76\u8cbb\u52a9\u6210\u4e8b\u696d\u306e\u52a9\u6210\u3092\u53d7\u3051\u3066\u884c\u308f\u308c\u307e\u3057\u305f\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u72d9\u3063\u305f\u5834\u6240\u306b\u30c9\u30ca\u30fcDNA\u3092\u633f\u5165\u3059\u308b\u30ce\u30c3\u30af\u30a4\u30f3\u65b0\u624b\u6cd5(BiPoD)\u3092\u958b\u767a 6%\uff08\u901a\u5e38\u6cd5\uff09\u304b\u308990%\u8d85\uff08\u65b0\u624b\u6cd5\uff09\u306b\u3000\u6b63\u78ba\u6027\u3068\u52b9\u7387\u304c\u5927\u5e45\u306b\u5411\u4e0a &nbsp; \u767a\u8868\u306e\u30dd\u30a4\u30f3\u30c8 \u5f93\u6765\u306e\u30ce\u30c3\u30af\u30a4\u30f3\u624b\u6cd5\u306f\u52b9\u7387\u306e\u4f4e\u3055\u3068\u3001\u610f\u56f3\u3057\u306a\u3044\u30c9\u30ca\u30fc [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":74940,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[40,1],"tags":[357,5],"class_list":["post-74930","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-topic","category-news","tag-pressrelease","tag-research"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/posts\/74930","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/comments?post=74930"}],"version-history":[{"count":2,"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/posts\/74930\/revisions"}],"predecessor-version":[{"id":74944,"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/posts\/74930\/revisions\/74944"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/media\/74940"}],"wp:attachment":[{"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/media?parent=74930"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/categories?post=74930"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/tags?post=74930"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}