{"id":39010,"date":"2016-03-17T16:39:00","date_gmt":"2016-03-17T07:39:00","guid":{"rendered":"http:\/\/www.waseda.jp\/top\/?p=39010"},"modified":"2016-03-17T16:48:33","modified_gmt":"2016-03-17T07:48:33","slug":"%e7%ad%8b%e7%ae%a1%e7%b4%b0%e8%83%9e%e3%81%ae%e6%b8%a9%e5%ba%a6%e5%a4%89%e5%8c%96%e3%82%92%e8%9b%8d%e5%85%89%e3%82%a4%e3%83%a1%e3%83%bc%e3%82%b8%e3%83%b3%e3%82%b0%e3%81%a7%e8%a8%88%e6%b8%ac%ef%bc%9a","status":"publish","type":"post","link":"https:\/\/www.waseda.jp\/top\/news\/39010","title":{"rendered":"\u7b4b\u7ba1\u7d30\u80de\u306e\u6e29\u5ea6\u5909\u5316\u3092\u86cd\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3067\u8a08\u6e2c \u71b1\u7523\u751f\u304c\u95a2\u308f\u308b\u30e1\u30bf\u30dc\u30ea\u30c3\u30af\u30b7\u30f3\u30c9\u30ed\u30fc\u30e0\u7b49\u306e\u6cbb\u7642\u306b\u671f\u5f85"},"content":{"rendered":"<h4 style=\"text-align: center;\">\u7b4b\u7ba1\u7d30\u80de\u306e\u6e29\u5ea6\u5909\u5316\u3092\u86cd\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3067\u8a08\u6e2c\uff1a\u5b9f\u9a13\u3068\u8a08\u7b97\u3068\u306e\u9593\u306e\u77db\u76fe\u89e3\u6d88\u3078<br \/>\n\u71b1\u7523\u751f\u304c\u95a2\u308f\u308b\u60aa\u6027\u9ad8\u71b1\u75c7\u3084\u30e1\u30bf\u30dc\u30ea\u30c3\u30af\u30b7\u30f3\u30c9\u30ed\u30fc\u30e0\u7b49\u306e\u6cbb\u7642\u306b\u671f\u5f85<\/h4>\n<p>\u65e9\u7a32\u7530\u5927\u5b66\u7406\u5de5\u5b66\u8853\u9662<a href=\"http:\/\/www.ishiwata.phys.waseda.ac.jp\/\" target=\"_blank\">\u77f3\u6e21\u4fe1\u4e00\uff08\u3044\u3057\u308f\u305f\u3057\u3093\u3044\u3061\uff09\u6559\u6388<\/a>\uff08\u65e9\u7a32\u7530\u5927\u5b66\u30d0\u30a4\u30aa\u30b5\u30a4\u30a8\u30f3\u30b9\u30b7\u30f3\u30ac\u30dd\u30fc\u30eb\u7814\u7a76\u6240\u9577\uff09\u3001\u91cd\u70b9\u9818\u57df\u7814\u7a76\u6a5f\u69cb<a href=\"https:\/\/researchers.waseda.jp\/profile\/ja.bd8b149aa39842dc589033e495116d90.html\" target=\"_blank\">\u9234\u6728\u56e3\uff08\u3059\u305a\u304d\u307e\u3069\u304b\uff09\u4e3b\u4efb\u7814\u7a76\u54e1\uff08\u7814\u7a76\u9662\u51c6\u6559\u6388\uff09<\/a>\u3001\u5927\u5b66\u9662\u5148\u9032\u7406\u5de5\u5b66\u7814\u7a76\u79d1\u535a\u58eb\u8ab2\u7a0b\uff14\u5e74\u4f0a\u85e4\u79c0\u57ce\uff08\u3044\u3068\u3046\u3072\u3067\u304d\uff09\u6c0f\uff08\u30b7\u30f3\u30ac\u30dd\u30fc\u30eb\u79d1\u5b66\u6280\u8853\u7814\u7a76\u5e81\u533b\u5b66\u751f\u7269\u5b66\u7814\u7a76\u6240\u7814\u7a76\u5b98\uff09\u3001\u91cd\u70b9\u9818\u57df\u7814\u7a76\u6a5f\u69cb\u65b0\u4e95\u654f\uff08\u3042\u3089\u3044\u3055\u3068\u3057\uff09\u62db\u8058\u7814\u7a76\u54e1\u3089\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u4f4e\u5206\u5b50\u86cd\u5149\u6e29\u5ea6\u8a08<span lang=\"EN-US\">ER thermo yellow<\/span>\u3068\u3001\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306e\uff11\u3064\u3067\u3042\u308b<span lang=\"EN-US\">mCherry<\/span>\u306e\u86cd\u5149\u5bff\u547d\u306b\u3001\u6e29\u5ea6\u4f9d\u5b58\u6027\uff08\u6e29\u5ea6\u304c\u4e0a\u304c\u308b\u306b\u3064\u308c\u3066\u3001\u86cd\u5149\u5bff\u547d\u304c\u77ed\u304f\u306a\u308b\uff09\u304c\u3042\u308b\u3053\u3068\u3092\u767a\u898b\u3057\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3001\u9aa8\u683c\u7b4b\u69d8\u306b\u5206\u5316\u3057\u305f\u57f9\u990a\u7d30\u80de\uff08\u7b4b\u7ba1\u7d30\u80de\uff09\u3092\u30ab\u30d5\u30a7\u30a4\u30f3\u3067\u523a\u6fc0\u3057\u305f\u969b\u306e\u3001\u7b4b\u5c0f\u80de\u4f53\u306b\u304a\u3051\u308b<span lang=\"EN-US\">ER thermo yello<\/span><span lang=\"EN-US\">w<\/span>\u3084\u3001\u7d30\u80de\u8cea\u306b\u304a\u3044\u3066\u767a\u73fe\u3057\u305f<span lang=\"EN-US\">mCherry<\/span>\u306e\u86cd\u5149\u5bff\u547d\u5909\u5316\u3092\u89b3\u5bdf\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u305d\u308c\u305e\u308c\u306e\u7d30\u80de\u5c0f\u5668\u5b98\u306b\u304a\u3051\u308b\u6e29\u5ea6\u5909\u5316\u3092\u6e2c\u5b9a\u3057\u307e\u3057\u305f\u3002\u7b4b\u7ba1\u7d30\u80de\u306e\u7b4b\u5c0f\u80de\u4f53\u3067\u6e29\u5ea6\u4e0a\u6607\u304c\u898b\u3089\u308c\u307e\u3057\u305f\u304c\u3001\u7d30\u80de\u8cea\u3067\u306f\u5927\u304d\u306a\u6e29\u5ea6\u5909\u5316\u304c\u898b\u3089\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u3053\u306e\u7d50\u679c\u306f\u3001\u9aa8\u683c\u7b4b\u3067\u306e\u975e\u3075\u308b\u3048\u71b1\u7523\u751f\u306b\u3088\u308b\u6e29\u5ea6\u5909\u5316\u304c\u3001\u7b4b\u5c0f\u80de\u4f53\u8fd1\u508d\u306e\u6975\u3081\u3066\u5c40\u6240\u3067\u8d77\u3053\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u6211\u3005\u30d2\u30c8\u3092\u542b\u3080\u591a\u304f\u306e\u751f\u7269\u306f\u3001\u7d76\u3048\u305a\u71b1\u3092\u7523\u751f\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u307e\u3067\u306b\u3001\u7d30\u80de\u30ec\u30d9\u30eb\u3067\u306e\u3055\u307e\u3056\u307e\u306a\u30bf\u30a4\u30d7\u306e\u6e29\u5ea6\u6e2c\u5b9a\u5b9f\u9a13\u3067\u3001\u5316\u5b66\u7269\u8cea\u306b\u3088\u308b\u7d30\u80de\u3078\u306e\u523a\u6fc0\u304c\u3001\uff11\u2103\u7a0b\u5ea6\u306e\u6e29\u5ea6\u4e0a\u6607\u3092\u5f15\u304d\u8d77\u3053\u3059\u3053\u3068\u304c\u5831\u544a\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u4e00\u65b9\u3067\u3001\u3053\u308c\u306f\u5148\u884c\u7814\u7a76\u3067\u3042\u308b\u71b1\u91cf\u6e2c\u5b9a\u88c5\u7f6e\u3067\u3042\u308b\u30de\u30a4\u30af\u30ed\u30ab\u30ed\u30ea\u30e1\u30c8\u30ea\u30fc\u306b\u3088\u308b\u7d30\u80de\u61f8\u6fc1\u6db2\u306e\u767a\u71b1\u91cf\u6e2c\u5b9a\u7d50\u679c\u304b\u3089\u6c42\u3081\u3089\u308c\u308b\u3001\u4e00\u7d30\u80de\u5f53\u305f\u308a\u306e\u6e29\u5ea6\u4e0a\u6607\u306e\u8a08\u7b97\u5024<span lang=\"EN-US\">10<sup>-5<\/sup> <\/span>\u2103\u3068\u5927\u304d\u306a\u9694\u305f\u308a\u304c\u3042\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u767a\u71b1\u91cf\u306f\u7d30\u80de\u306e\u7a2e\u985e\u306b\u3088\u3063\u3066\u5dee\u304c\u3042\u3063\u305f\u308a\u3001\u7d30\u80de\u5168\u4f53\u306b\u6e29\u5ea6\u4e0a\u6607\u304c\u306a\u3044\u3068\u4eee\u5b9a\u3057\u305f\u308a\u3059\u308c\u3070\u3001\u3053\u306e\u30ae\u30e3\u30c3\u30d7\u306f\u89e3\u6d88\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u672c\u7814\u7a76\u306f\u3001\u7d30\u80de\u5c0f\u5668\u5b98\u3054\u3068\u306b\u6e29\u5ea6\u4e0a\u6607\u306e\u3070\u3089\u3064\u304d\u304c\u3042\u308b\u3053\u3068\u3092\u660e\u3089\u304b\u306b\u3057\u3001\u3053\u306e<span lang=\"EN-US\">10<sup>5<\/sup><\/span>\u30ae\u30e3\u30c3\u30d7\u306e\u89e3\u6d88\u3078\u4e00\u77f3\u3092\u6295\u3058\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3057\u305f\u3002<\/p>\n<p>\u3055\u3089\u306b\u3001\u7d30\u80de\u5c0f\u5668\u5b98\u30ec\u30d9\u30eb\u3067\u6e29\u5ea6\u5909\u5316\u3092\u6e2c\u5b9a\u3067\u304d\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u305f\u3081\u3001\u3053\u306e\u624b\u6cd5\u3092\u5fdc\u7528\u3057\u3066\u3001\u4eca\u5f8c\u3001\u9aa8\u683c\u7b4b\u306b\u304a\u3051\u308b\u975e\u3075\u308b\u3048\u71b1\u7523\u751f\u306b\u81ea\u4f53\u306e\u7814\u7a76\u3092\u9032\u3081\u308b\u3060\u3051\u3067\u306a\u304f\u3001\u71b1\u7523\u751f\u304c\u95a2\u308f\u3063\u3066\u3044\u308b\u60aa\u6027\u9ad8\u71b1\u75c7\u3084\u30e1\u30bf\u30dc\u30ea\u30c3\u30af\u30b7\u30f3\u30c9\u30ed\u30fc\u30e0\u306a\u3069\u306e\u75be\u60a3\u306e\u6cbb\u7642\u3001\u85ac\u5264\u8a55\u4fa1\u306b\u8ca2\u732e\u3059\u308b\u3053\u3068\u304c\u671f\u5f85\u3055\u308c\u307e\u3059\u3002<\/p>\n<p>\u4eca\u56de\u306e\u7814\u7a76\u6210\u679c\u306f\u3001\u82f1\u56fd\u738b\u7acb\u5316\u5b66\u4f1a\uff08<span lang=\"EN-US\">Royal Society of Chemistry<\/span>\uff09\u306e\u79d1\u5b66\u96d1\u8a8c\u300e<span lang=\"EN-US\">Chemical Communications<\/span>\u300f\u30aa\u30f3\u30e9\u30a4\u30f3\u7248\u306b\uff11\u6708\uff12\uff11\u65e5\uff08\u73fe\u5730\u6642\u9593\uff09\u306b\u63b2\u8f09\u3055\u308c\u3001<span lang=\"EN-US\">3<\/span>\u6708<span lang=\"EN-US\">15<\/span>\u65e5\u306e\u8a8c\u4e0a\u63b2\u8f09\u3067\u306f<a href=\"http:\/\/pubs.rsc.org\/en\/content\/articlelanding\/2016\/cc\/c6cc90132h#!divAbstract\" target=\"_blank\"><span lang=\"EN-US\">outside back cover<\/span><\/a>\u3068\u3057\u3066\u63a1\u629e\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n<p>\u8ad6\u6587\uff1a<a href=\"http:\/\/pubs.rsc.org\/en\/content\/articlelanding\/2016\/cc\/c5cc09943a#!divAbstract\" target=\"_blank\">Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes<\/a><\/p>\n<p>\u203b\u672c\u7814\u7a76\u306f\u3001\u30b7\u30f3\u30ac\u30dd\u30fc\u30eb\u79d1\u5b66\u6280\u8853\u7814\u7a76\u5e81\u3001\u30b7\u30f3\u30ac\u30dd\u30fc\u30eb\u56fd\u7acb\u5927\u5b66\u3068\u306e\u5171\u540c\u7814\u7a76\u3068\u3057\u3066\u5b9f\u65bd\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n<h5><span lang=\"EN-US\">(1)<\/span>\u3053\u308c\u307e\u3067\u306e\u7814\u7a76\u3067\u5206\u304b\u3063\u3066\u3044\u305f\u3053\u3068\uff08\u79d1\u5b66\u53f2\u7684\u30fb\u6b74\u53f2\u7684\u306a\u80cc\u666f\u306a\u3069\uff09<\/h5>\n<p>\u6211\u3005\u30d2\u30c8\u3092\u542b\u3080\u591a\u304f\u306e\u751f\u7269\u306f\u3001\u7d76\u3048\u305a\u71b1\u3092\u7523\u751f\u3057\u3066\u3044\u307e\u3059\u3002\u6c17\u6e29\u304c\u5909\u308f\u3063\u305f\u3068\u304d\u306a\u3069\u3001\u5916\u90e8\u306e\u74b0\u5883\u306b\u9069\u5fdc\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u5927\u304d\u306a\u71b1\u7523\u751f\u304c\u5fc5\u8981\u3068\u306a\u308a\u307e\u3059\u3002\u305d\u306e\u4e3b\u306a\u767a\u71b1\u6e90\u3068\u3057\u3066\u3001\u8910\u8272\u8102\u80aa\u7d44\u7e54\u3084\u9aa8\u683c\u7b4b\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u9aa8\u683c\u7b4b\u3067\u306f\u3001\u3075\u308b\u3048\u71b1\u7523\u751f\u304c\u4e3b\u306a\u767a\u71b1\u30e1\u30ab\u30cb\u30ba\u30e0\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3057\u305f\u304c\u3001\u8fd1\u5e74\u3001\u975e\u3075\u308b\u3048\u71b1\u7523\u751f\u3082\u767a\u751f\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u5831\u544a\u3055\u308c\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u3053\u308c\u307e\u3067\u306e\u9aa8\u683c\u7b4b\u306b\u304a\u3051\u308b\u975e\u3075\u308b\u3048\u71b1\u7523\u751f\u306b\u95a2\u3059\u308b\u7814\u7a76\u306f\u3001\u500b\u4f53\u30ec\u30d9\u30eb<span lang=\"EN-US\">(1)<\/span>\u3082\u3057\u304f\u306f\u5206\u5b50\u30ec\u30d9\u30eb<span lang=\"EN-US\">(2)<\/span>\u3067\u3042\u3063\u305f\u305f\u3081\u3001\u4e21\u8005\u306e\u6a4b\u6e21\u3057\u3092\u3059\u308b\u7d30\u80de\u30ec\u30d9\u30eb\u3067\u306e\u7814\u7a76\u304c\u5fc5\u8981\u3068\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u7d30\u80de\u30ec\u30d9\u30eb\u3067\u306e\u6e29\u5ea6\u6e2c\u5b9a\u624b\u6cd5\u306f\u3001\u672c\u7814\u7a76\u30c1\u30fc\u30e0\u304b\u3089\u30d4\u30da\u30c3\u30c8\u578b<span lang=\"EN-US\">(3)<\/span>\u3001\u30b7\u30fc\u30c8\u578b<span lang=\"EN-US\">(4)<\/span>\u3001\u30ca\u30ce\u7c92\u5b50<span lang=\"EN-US\">(5,6)<\/span>\u304c\u767a\u8868\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u4ed6\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u304b\u3089\u3082\u3001\u3055\u307e\u3056\u307e\u306a\u30bf\u30a4\u30d7\u306e\u6e29\u5ea6\u6e2c\u5b9a\u624b\u6cd5\u304c\u958b\u767a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u3046\u3057\u305f\u6e29\u5ea6\u6e2c\u5b9a\u624b\u6cd5\u3067\u3001\u5316\u5b66\u7269\u8cea\u306b\u3088\u308b\u7d30\u80de\u3078\u306e\u523a\u6fc0\u304c\u3001\uff11\u2103\u7a0b\u5ea6\u306e\u6e29\u5ea6\u4e0a\u6607\u3092\u5f15\u304d\u8d77\u3053\u3059\u3053\u3068\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u7d50\u679c\u306f\u73fe\u5728\u3001\u8b70\u8ad6\u3092\u5f15\u304d\u8d77\u3053\u3057\u3066\u3044\u307e\u3059<span lang=\"EN-US\">(7<\/span>\u2013<span lang=\"EN-US\">10)<\/span>\u3002\u3068\u3044\u3046\u306e\u306f\u3001\u5148\u884c\u7814\u7a76\u306b\u304a\u3044\u3066\u71b1\u91cf\u6e2c\u5b9a\u88c5\u7f6e\u3067\u3042\u308b\u30de\u30a4\u30af\u30ed\u30ab\u30ed\u30ea\u30e1\u30c8\u30ea\u30fc\u306b\u3088\u308b\u7d30\u80de\u61f8\u6fc1\u6db2\u306e\u767a\u71b1\u91cf\u6e2c\u5b9a\u304c\u884c\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u7d50\u679c\u304b\u3089\u8a08\u7b97\u306b\u3088\u3063\u3066\u6c42\u3081\u3089\u308c\u308b\u6e29\u5ea6\u4e0a\u6607\u306f\uff11\u7d30\u80de\u5f53\u305f\u308a<span lang=\"EN-US\">10<sup>-5<\/sup><\/span>\u2103\u7a0b\u5ea6\u3067\u3042\u308b\u3068\u63a8\u5b9a\u3055\u308c\u3066\u3044\u308b\u304b\u3089\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u767a\u71b1\u91cf\u306f\u7d30\u80de\u306e\u7a2e\u985e\u306b\u3088\u3063\u3066\u5927\u304d\u306a\u5dee\u304c\u3042\u3063\u305f\u308a\u3001\u7d30\u80de\u5168\u4f53\u306b\u6e29\u5ea6\u4e0a\u6607\u304c\u306a\u3044\u3068\u4eee\u5b9a\u3057\u305f\u308a\u3059\u308c\u3070\u3001\u3053\u306e\u30ae\u30e3\u30c3\u30d7\u306f\u89e3\u6d88\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<h5><span lang=\"EN-US\">(2)<\/span>\u4eca\u56de\u306e\u7814\u7a76\u3067\u65b0\u305f\u306b\u5b9f\u73fe\u3057\u3088\u3046\u3068\u3057\u305f\u3053\u3068\u3001\u660e\u3089\u304b\u306b\u306a\u3063\u305f\u3053\u3068<\/h5>\n<p><span lang=\"EN-US\">C2C12<\/span>\u7d30\u80de\u3068\u3044\u3046\u9aa8\u683c\u7b4b\u69d8\u306b\u5206\u5316\u3059\u308b\u57f9\u990a\u7d30\u80de\u306b\u5916\u90e8\u304b\u3089\u523a\u6fc0\u3092\u52a0\u3048\u305f\u3068\u304d\u306b\u3001\u7d30\u80de\u5185\u3067\u3069\u306e\u3088\u3046\u306a\u6e29\u5ea6\u5909\u5316\u304c\u8d77\u304d\u308b\u306e\u304b\u3092\u6e2c\u5b9a\u3057\u307e\u3057\u305f\u3002<\/p>\n<h5><span lang=\"EN-US\">(3)<\/span>\u305d\u306e\u305f\u3081\u306b\u65b0\u3057\u304f\u958b\u767a\u3057\u305f\u624b\u6cd5<\/h5>\n<p>\u7d30\u80de\u5c0f\u5668\u5b98\u3067\u3042\u308b\u5c0f\u80de\u4f53\u306b\u5c40\u5728\u3059\u308b\u4f4e\u5206\u5b50\u86cd\u5149\u6e29\u5ea6\u8a08<span lang=\"EN-US\">ER thermo yellow(11)<\/span>\u3092\u7528\u3044\u3066\u3001\u9aa8\u683c\u7b4b\u306b\u304a\u3051\u308b\u71b1\u7523\u751f\u304c\u884c\u308f\u308c\u308b\u5834\u6240\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u308b\u7b4b\u5c0f\u80de\u4f53\u306e\u6e29\u5ea6\u5909\u5316\u3092\u6e2c\u5b9a\u3057\u307e\u3057\u305f\uff08\u56f3\uff11\uff09\u3002\u307e\u305f\u3001\u7d30\u80de\u8cea\u306b\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u306e\uff11\u3064\u3067\u3042\u308b<span lang=\"EN-US\">mCherry<\/span>\u3092\u767a\u73fe\u3055\u305b\u3001\u7d30\u80de\u8cea\u5185\u306e\u6e29\u5ea6\u5909\u5316\u3082\u6e2c\u5b9a\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u6e2c\u5b9a\u306f\u3001\u86cd\u5149\u5bff\u547d\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u9855\u5fae\u93e1\u6cd5\uff08<span lang=\"EN-US\">FLIM<\/span>\uff09\u3092\u7528\u3044\u3066\u884c\u308f\u308c\u307e\u3057\u305f\u3002<span lang=\"EN-US\">FLIM<\/span>\u306f\u86cd\u5149\u7269\u8cea\u306e\u6fc3\u5ea6\u5909\u5316\u3084\u6e2c\u5b9a\u88c5\u7f6e\u306e\u5f71\u97ff\u3092\u53d7\u3051\u3065\u3089\u3044\u306a\u3069\u3001\u901a\u5e38\u7528\u3044\u3089\u308c\u308b\u86cd\u5149\u5f37\u5ea6\u306e\u6e2c\u5b9a\u3068\u6bd4\u3079\u3001\u6b63\u78ba\u306a\u7d50\u679c\u304c\u5f97\u3089\u308c\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059<span lang=\"EN-US\">(12)<\/span>\u3002<\/p>\n<p style=\"text-align: center;\">\u00a0<img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-39011 aligncenter\" alt=\"20160316_fig1\" src=\"http:\/\/www.waseda.jp\/top\/assets\/uploads\/2016\/03\/20160316_fig1.png\" width=\"582\" height=\"260\" \/><\/p>\n<p align=\"center\"><strong>\u56f3\uff11\uff0e\u4f4e\u5206\u5b50\u86cd\u5149\u6e29\u5ea6\u8a08ER thermo yellow\u3068\u6e29\u5ea6\u4f9d\u5b58\u7684\u306a\u86cd\u5149\u5bff\u547d\u5909\u5316\u306e\u6982\u5ff5\u56f3<\/strong><\/p>\n<p><strong>ER thermo yellow\u3092\u7d30\u80de\u57f9\u990a\u6db2\u306b\u6dfb\u52a0\u3059\u308b\u3068\u3001\u7d30\u80de\u5185\u306e\u5c0f\u80de\u4f53\uff08\u7b4b\u7ba1\u7d30\u80de\u306e\u5834\u5408\u306f\u7b4b\u5c0f\u80de\u4f53\uff09\u306b\u5c40\u5728\u3059\u308b\u3002\u86cd\u5149\u306b\u3088\u308b\u5149\u5b50\u653e\u51fa\u91cf\u306f\u3001\u52b1\u8d77\u5f8c\u306e\u6642\u9593\u306b\u6cbf\u3063\u3066\u6307\u6570\u95a2\u6570\u7684\u306b\u6e1b\u8870\u3059\u308b\u3002\u6e29\u5ea6\u306b\u3088\u3063\u3066\u86cd\u5149\u306e\u6e1b\u8870\u5ea6\u5408\u3044\u304c\u5909\u5316\u3059\u308c\u3070\u3001\u9006\u306b\u86cd\u5149\u6e1b\u8870\u66f2\u7dda\u304b\u3089\u6e29\u5ea6\u304c\u6e2c\u5b9a\u3067\u304d\u308b\u3002<\/strong><\/p>\n<h5>(4)\u4eca\u56de\u306e\u7814\u7a76\u3067\u5f97\u3089\u308c\u305f\u7d50\u679c\u53ca\u3073\u77e5\u898b<\/h5>\n<p>\u307e\u305a\u3001ER thermo yellow\u306e\u86cd\u5149\u5bff\u547d\u306b\u6e29\u5ea6\u4f9d\u5b58\u6027\u304c\u3042\u308b\u3053\u3068\u3092\u767a\u898b\u3057\u307e\u3057\u305f\uff08\u56f3\uff12\uff09\u3002\u305d\u3053\u3067\u3001\u5206\u5316\u3057\u305fC2C12\u7d30\u80de\uff08\u7b4b\u7ba1\u7d30\u80de\uff09\u306bER thermo yellow\u3092\u5c0e\u5165\u3057\u3066\u3001\u7b4b\u5c0f\u80de\u4f53\u306e\u6e29\u5ea6\u5909\u5316\u3092\u6e2c\u5b9a\u3057\u307e\u3057\u305f\u3002Caffeine\uff08\u30ab\u30d5\u30a7\u30a4\u30f3\uff09\u306b\u3088\u3063\u3066\u5916\u90e8\u523a\u6fc0\u3092\u4e0e\u3048\u308b\u3068\u3001\u7b4b\u5c0f\u80de\u4f53\u306b\u3042\u308b\u30ea\u30a2\u30ce\u30b8\u30f3\u53d7\u5bb9\u4f53\u304b\u3089\u30ab\u30eb\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\u653e\u51fa\u304c\u8d77\u3053\u308a\u3001\u305d\u306e\u30ab\u30eb\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\u3092\u7b4b\u5c0f\u80de\u4f53\u306e\u30ab\u30eb\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\u30dd\u30f3\u30d7\u3067\u3042\u308bSERCA\uff08sarco\/endoplasmic reticulum Ca<sup>2+<\/sup>-ATPase\uff09\u304c\u56de\u53ce\u3059\u308b\u904e\u7a0b\uff08\u56f3\uff13\uff09\u306b\u304a\u3044\u3066\u3001\u6e29\u5ea6\u4e0a\u6607\u304c\u89b3\u3089\u308c\u307e\u3057\u305f\uff08~1.6\u2103\uff09\u3002\u4e00\u65b9\u3001thapsigargin\uff08\u30bf\u30d7\u30b7\u30ac\u30eb\u30ae\u30f3\uff09\u3067\u523a\u6fc0\u3057\u305f\u5834\u5408\u3001\u30ab\u30d5\u30a7\u30a4\u30f3\u523a\u6fc0\u3068\u540c\u69d8\u306b\u3001\u7d30\u80de\u8cea\u306e\u30ab\u30eb\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\u6fc3\u5ea6\u306f\u4e0a\u6607\u3057\u307e\u3057\u305f\u304c\u3001\u5927\u304d\u306a\u6e29\u5ea6\u5909\u5316\u306f\u89b3\u3089\u308c\u307e\u305b\u3093\u3067\u3057\u305f\uff08~0.38\u2103\uff09\u3002\u3053\u308c\u306f\u3001\u30bf\u30d7\u30b7\u30ac\u30eb\u30ae\u30f3\u304cSERCA\u3092\u963b\u5bb3\u3057\u305f\u305f\u3081\u3067\u3001SERCA\u304c\u71b1\u3092\u7523\u751f\u3059\u308b\u3068\u3044\u3046\u5f93\u6765\u304b\u3089\u306e\u8aac\u3092\u88cf\u4ed8\u3051\u308b\u7d50\u679c\u3067\u3059\u3002<\/p>\n<p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-39012 aligncenter\" alt=\"20160316_fig2\" src=\"http:\/\/www.waseda.jp\/top\/assets\/uploads\/2016\/03\/20160316_fig2.png\" width=\"370\" height=\"345\" \/><\/p>\n<p align=\"center\"><strong>\u56f3\uff12\uff0eER thermo yellow\u86cd\u5149\u5bff\u547d\u306e\u6e29\u5ea6\u4f9d\u5b58\u6027<\/strong><\/p>\n<p><strong>\u7b4b\u7ba1\u7d30\u80de\u5185\u306eER thermo yellow\u306e\u86cd\u5149\u5bff\u547d\u3068\u6e29\u5ea6\u306e\u95a2\u4fc2\uff08\u9ed2\u4e38\uff09\u3002\u86cd\u5149\u5bff\u547d\u306f\u6e2c\u5b9a\u7bc4\u56f2\u5185\u3067\u6e29\u5ea6\u306b\u5bfe\u3057\u3066\u76f4\u7dda\u7684\u306a\u5909\u5316\u3092\u793a\u3057\u305f\uff08\u8fd1\u4f3c\u76f4\u7dda\u306e\u50be\u304d\uff1a-24 \u30d4\u30b3\u79d2\/\u2103\uff09\u3002\u6e29\u5ea6\u6e2c\u5b9a\u7cbe\u5ea6\u306f\u767d\u4e38\u306e\u3088\u3046\u306b\u6c42\u3081\u3089\u308c\u305f\uff08\u5e73\u5747\u5024\uff1a1.3 \u00b1 0.5\u2103\uff09\u3002<\/strong><\/p>\n<p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-39013 aligncenter\" alt=\"20160316_fig3\" src=\"http:\/\/www.waseda.jp\/top\/assets\/uploads\/2016\/03\/20160316_fig3.png\" width=\"517\" height=\"295\" \/><\/p>\n<p align=\"center\"><strong>\u56f3\uff13\uff0e\u9aa8\u683c\u7b4b\u306b\u304a\u3051\u308b\u767a\u71b1\u30e1\u30ab\u30cb\u30ba\u30e0<\/strong><\/p>\n<p><strong>\u30ab\u30d5\u30a7\u30a4\u30f3\u306f\u7b4b\u5c0f\u80de\u4f53\u4e0a\u306e\u30ea\u30a2\u30ce\u30b8\u30f3\u53d7\u5bb9\u4f53\u304b\u3089\u306e\u30ab\u30eb\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\u653e\u51fa\u3092\u5f15\u304d\u8d77\u3053\u3059\u3002SERCA\u304c\u7b4b\u5c0f\u80de\u4f53\u306b\u30ab\u30eb\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\u3092\u53d6\u308a\u8fbc\u3080\u969b\u3001SERCA\u306e\u7a7a\u56de\u308a\u306b\u3088\u308a\u71b1\u304c\u751f\u3058\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u308b\u3002\u30bf\u30d7\u30b7\u30ac\u30eb\u30ae\u30f3\u306fSERCA\u3092\u963b\u5bb3\u3059\u308b\u85ac\u5264\u3067\u3001\u7d30\u80de\u8cea\uff0d\u7b4b\u5c0f\u80de\u4f53\u9593\u306e\u30ab\u30eb\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\u6d41\u304c\u906e\u65ad\u3055\u308c\u308b\u3053\u3068\u3067\u3001\u7d30\u80de\u8cea\u5185\u306e\u30ab\u30eb\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\u6fc3\u5ea6\u304c\u4e0a\u6607\u3059\u308b\u3002<\/strong><\/p>\n<p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-medium wp-image-39015\" alt=\"20160316_fig4\" src=\"http:\/\/www.waseda.jp\/top\/assets\/uploads\/2016\/03\/20160316_fig4-610x241.png\" width=\"610\" height=\"241\" srcset=\"https:\/\/www.waseda.jp\/top\/assets\/uploads\/2016\/03\/20160316_fig4-610x241.png 610w, https:\/\/www.waseda.jp\/top\/assets\/uploads\/2016\/03\/20160316_fig4.png 918w\" sizes=\"auto, (max-width: 610px) 100vw, 610px\" \/><\/p>\n<p align=\"center\"><strong>\u56f3\uff14\uff0e\u30ab\u30d5\u30a7\u30a4\u30f3\u53ca\u3073\u30bf\u30d7\u30b7\u30ac\u30eb\u30ae\u30f3\u523a\u6fc0\u524d\u5f8c\u306eER thermo yellow\u86cd\u5149\u5bff\u547d\u306e\u6642\u9593\u5909\u5316<\/strong><\/p>\n<p><strong>\u30ab\u30d5\u30a7\u30a4\u30f3\u523a\u6fc0\u5f8c\u3001ER thermo 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C. Bal, S. K. Maurya, D. H. Sopariwala, S. K. Sahoo, S. C. Gupta, S. A. Shaikh, M. Pant, L. A. Rowland, S. A. Goonasekera, J. D. Molkentin and M. Periasamy, Nat. Med., 2012, 18, 1575\u20131579.<\/li>\n<li>2. \u00a0\u00a0\u00a0\u00a0 L. de Meis, J. Biol. Chem., 2001, 276, 25078\u201325087.<\/li>\n<li>3. \u00a0\u00a0\u00a0\u00a0 V. Zeeb, M. Suzuki, S. Ishiwata, J. Neurosci. Methods, 2004, 139, 69\u201377.<\/li>\n<li>4. \u00a0\u00a0\u00a0\u00a0 H. Itoh, K. Oyama, M. Suzuki and S. Ishiwata, BIOPHYSICS, 2014, 10, 109\u2013119.<\/li>\n<li>5. \u00a0\u00a0\u00a0\u00a0 K. Oyama, M. Takabayashi, Y. Takei, S. Arai, S. Takeoka, S. Ishiwata and M. Suzuki, Lab Chip, 2012, 12, 1591\u20131593.<\/li>\n<li>6. \u00a0\u00a0\u00a0\u00a0 Y. Takei, S. Arai, A. Murata, M. Takabayashi, K. Oyama, S. Ishiwata, S. Takeoka and M. Suzuki, ACS Nano, 2014, 8, 198\u2013206.<\/li>\n<li>7. \u00a0\u00a0\u00a0\u00a0 G. Baffou, H. Rigneault, D. Marguet and L. Jullien, Nat. Methods, 2014, 11, 899\u2013901.<\/li>\n<li>8. \u00a0\u00a0\u00a0\u00a0 S. Kiyonaka, R. Sakaguchi, I. Hamachi, T. Morii, T. Yoshizaki and Y. Mori, Nat. Methods, 2015, 12, 801\u2013802.<\/li>\n<li>9. \u00a0\u00a0\u00a0\u00a0 M. Suzuki, V. Zeeb, S. Arai, K. Oyama and S. Ishiwata, Nat. Methods, 2015, 12, 802\u2013803.<\/li>\n<li>10. \u00a0\u00a0 G. Baffou, H. Rigneault, D. Marguet and L. Jullien, Nat. Methods, 2015, 12, 803\u2013803.<\/li>\n<li>11. \u00a0\u00a0 S. Arai, S.-C. Lee, D. Zhai, M. Suzuki and Y. T. Chang, Sci. Rep., 2014, 4, 6701.<\/li>\n<li>12. \u00a0\u00a0 X. Wang, O. S. Wolfbeis and R. J. Meier, Chem. Soc. Rev., 2013, 42, 7834\u20137869.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7b4b\u7ba1\u7d30\u80de\u306e\u6e29\u5ea6\u5909\u5316\u3092\u86cd\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3067\u8a08\u6e2c\uff1a\u5b9f\u9a13\u3068\u8a08\u7b97\u3068\u306e\u9593\u306e\u77db\u76fe\u89e3\u6d88\u3078 \u71b1\u7523\u751f\u304c\u95a2\u308f\u308b\u60aa\u6027\u9ad8\u71b1\u75c7\u3084\u30e1\u30bf\u30dc\u30ea\u30c3\u30af\u30b7\u30f3\u30c9\u30ed\u30fc\u30e0\u7b49\u306e\u6cbb\u7642\u306b\u671f\u5f85 \u65e9\u7a32\u7530\u5927\u5b66\u7406\u5de5\u5b66\u8853\u9662\u77f3\u6e21\u4fe1\u4e00\uff08\u3044\u3057\u308f\u305f\u3057\u3093\u3044\u3061\uff09\u6559\u6388\uff08\u65e9\u7a32\u7530\u5927\u5b66\u30d0\u30a4\u30aa\u30b5\u30a4\u30a8\u30f3\u30b9 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":39013,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[40,1],"tags":[5],"class_list":["post-39010","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-topic","category-news","tag-research"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/posts\/39010","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/comments?post=39010"}],"version-history":[{"count":1,"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/posts\/39010\/revisions"}],"predecessor-version":[{"id":39017,"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/posts\/39010\/revisions\/39017"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/media\/39013"}],"wp:attachment":[{"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/media?parent=39010"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/categories?post=39010"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.waseda.jp\/top\/wp-json\/wp\/v2\/tags?post=39010"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}