A team of researchers from Waseda University, the University of Tokyo, the National University of Singapore, and Harvard University used single fluorescent proteins to develop multicolor adenosine triphospate (ATP) indicators, available in red, green, and blue (RGB-color) hues. The multicolor ATP indicators enable the simultaneous visualization of subcellular ATP dynamics in the cytoplasm and mitochondria of cells derived from mammals, plants, and worms.
“By further expanding the usability of the indicators, this accessible technology may be applied by researchers to facilitate the study of underlying metabolic processes in health and disease across both animal and plant kingdoms,” says Satoshi Arai, a junior researcher and assistant professor of analytical chemistry at Waseda University.
This study was published online in Angewandte Chemie International Edition on June 28, 2018.
- Article title: RGB-color intensiometric indicators to visualize spatiotemporal dynamics of ATP in single cells
- Authors: Satoshi Arai, Rókus Kriszt, Kazuki Harada, Liang-Sheng Looi, Shogo Matsuda, Devina Wongso, Satoshi Suo, Shoichi Ishiura, Yu-Hua Tseng, Michael Raghunath, Toshiro Ito, Takashi Tsuboi, Tetsuya Kitaguchi
- Published online in Angewandte Chemie International Edition on June 28, 2018