{"id":74021,"date":"2023-02-22T10:22:05","date_gmt":"2023-02-22T01:22:05","guid":{"rendered":"https:\/\/www.waseda.jp\/inst\/research\/?p=74021"},"modified":"2023-02-22T10:22:05","modified_gmt":"2023-02-22T01:22:05","slug":"neutrophil-depletion-attenuates-acute-liver-stress-after-exhaustive-exercice-in-mice%ef%bc%88published-in-medicine-and-science-in-sports-and-exercise-december-2022%ef%bc%89","status":"publish","type":"post","link":"https:\/\/www.waseda.jp\/inst\/research\/news\/74021","title":{"rendered":"Neutrophil Depletion Attenuates Acute Liver Stress after Exhaustive Exercice in Mice\uff08Published in Medicine and Science in Sports and Exercise, December 2022\uff09"},"content":{"rendered":"\n\n\n\n\n\n\n\n\n
Journal Title
\n\/\u63b2\u8f09\u30b8\u30e3\u30fc\u30ca\u30eb\u540d<\/td>\n		Medicine and Science in Sports and Exercise<\/td>\n<\/tr>\n
Publication Year and Month
\n\/\u63b2\u8f09\u5e74\u6708<\/td>\n		December, 2022<\/td>\n<\/tr>\n
Paper Title
\n\/\u8ad6\u6587\u30bf\u30a4\u30c8\u30eb<\/td>\n		Neutrophil Depletion Attenuates Acute Liver Stress after Exhaustive Exercice in Mice<\/td>\n<\/tr>\n
DOI
\n\/\u8ad6\u6587DOI<\/td>\n		10.1249\/MSS.0000000000003094<\/a><\/td>\n<\/tr>\n
\u00a0Author of Waseda University
\n\/\u672c\u5b66\u306e\u8457\u8005<\/td>\n		SUZUKI, Katsuhiko(Professor, Faculty of Sport Sciences, School of Sport and Sciences):Last Author<\/td>\n<\/tr>\n
Related Websites
\n\/\u95a2\u9023Web<\/td>\n		–<\/td>\n<\/tr>\n
Abstract
\n\/\u6284\u9332<\/td>\n		\n

Purpose<\/h3>\n

Exhaustive exercise induces acute liver stress; however, the precise mechanisms remain unclear.<\/p>\n

Methods<\/h3>\n

We investigated the effects of neutrophil depletion in male C57BL\/6 J mice. Male C57BL\/6 J mice were divided into four groups: sedentary with control antibody (n = 20), sedentary with antineutrophil antibody (n = 20), exhaustive exercise with control antibody (n = 20), and exhaustive exercise with antineutrophil antibody (n = 20). Antineutrophil antibodies (1A8) or control antibodies were administered intraperitoneally prior to running on a treadmill. Immediately and at 24 h after running to exhaustion on a treadmill at a 7% gradient and a speed of 24 m\/min, blood neutrophil counts were measured by flow cytometry. Plasma activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were also measured. HE staining was performed to calculate the liver stress score, and hepatic tumor necrosis factor (TNF)-? was measured using enzyme-linked immunosorbent assay (ELISA).<\/p>\n

Results<\/h3>\n

Exercise increased blood neutrophil and neutrophil infiltration into the liver. Plasma AST and ALT activities were significantly higher immediately after exhaustive exercise than after sedentary control (AST, sedentary with control antibody:52.2 \u00b1 .4.2, exhaustive exercise with control antibody:210.0 \u00b1 19.8, ALT, sedentary with control antibody:29.8 \u00b1 2.2, exhaustive exercise with control antibody:87.2 \u00b1 15.8). However, AST and ALT activities were significantly decreased with the 1A8 antibody (AST:102.2 \u00b1 12.9, ALT:39.2 \u00b1 4.0). In addition, the liver stress score increased after exercise but was significantly reduced by prior 1A8 antibody administration. The 1A8 antibody treatment also decreased hepatic TNF-\u03b1 levels after exhaustive exercise.<\/p>\n

Conclusions<\/h3>\n

These results suggested that neutrophils play a critical role in increasing liver stress by regulating inflammation.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Journal Title \/\u63b2\u8f09\u30b8\u30e3\u30fc\u30ca\u30eb\u540d Medicine and Science in Sports and Exercise Publication Year and Month \/\u63b2\u8f09\u5e74\u6708 December, […]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[],"tags":[218,217],"class_list":["post-74021","post","type-post","status-publish","format-standard","hentry","tag-impact-en","tag-impact"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts\/74021","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/comments?post=74021"}],"version-history":[{"count":1,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts\/74021\/revisions"}],"predecessor-version":[{"id":74028,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts\/74021\/revisions\/74028"}],"wp:attachment":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/media?parent=74021"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/categories?post=74021"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/tags?post=74021"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}