{"id":73932,"date":"2023-01-12T15:25:22","date_gmt":"2023-01-12T06:25:22","guid":{"rendered":"https:\/\/www.waseda.jp\/inst\/research\/?p=73932"},"modified":"2023-01-12T15:25:22","modified_gmt":"2023-01-12T06:25:22","slug":"targeted-single-cell-genomics-reveals-novel-host-adaptation-strategies-of-the-symbiotic-bacteria-endozoicomonas-in-acropora-tenuis-coral%ef%bc%88published-in-microbiome-december-2022%ef%bc%89","status":"publish","type":"post","link":"https:\/\/www.waseda.jp\/inst\/research\/news\/73932","title":{"rendered":"Targeted single-cell genomics reveals novel host adaptation strategies of the symbiotic bacteria Endozoicomonas in Acropora tenuis coral\uff08Published in Microbiome, December 2022\uff09"},"content":{"rendered":"\n\n\n\n\n\n\n\n\n
Journal Title
\n\/\u63b2\u8f09\u30b8\u30e3\u30fc\u30ca\u30eb\u540d<\/td>\n		Microbiome<\/td>\n<\/tr>\n
Publication Year and Month
\n\/\u63b2\u8f09\u5e74\u6708<\/td>\n		December, 2022<\/td>\n<\/tr>\n
Paper Title
\n\/\u8ad6\u6587\u30bf\u30a4\u30c8\u30eb<\/td>\n		Targeted single-cell genomics reveals novel host adaptation strategies of the symbiotic bacteria Endozoicomonas in Acropora tenuis coral<\/td>\n<\/tr>\n
DOI
\n\/\u8ad6\u6587DOI<\/td>\n		10.1186\/s40168-022-01395-9<\/a><\/td>\n<\/tr>\n
\u00a0Author of Waseda University
\n\/\u672c\u5b66\u306e\u8457\u8005<\/td>\n		TAKEYAMA, Haruko(Professor, Faculty of Science and Engineering, School of Advanced Science and Engineering):Corresponding Author<\/td>\n<\/tr>\n
Related Websites
\n\/\u95a2\u9023Web<\/td>\n		–<\/td>\n<\/tr>\n
Abstract
\n\/\u6284\u9332<\/td>\n		\n

Background<\/h3>\n

Endozoicomonas<\/i>\u00a0bacteria symbiosis with various marine organisms is hypothesized as a potential indicator of health in corals. Although many amplicon analyses using 16S rRNA gene have suggested the diversity of\u00a0Endozoicomonas<\/i>\u00a0species, genome analysis has been limited due to contamination of host-derived sequences and difficulties in culture and metagenomic analysis. Therefore, the evolutionary and functional potential of individual\u00a0Endozoicomonas<\/i>\u00a0species symbiotic with the same coral species remains unresolved.<\/p>\n

Results<\/h3>\n

In this study, we applied a novel single-cell genomics technique using droplet microfluidics to obtain single-cell amplified genomes (SAGs) for uncultured coral-associated\u00a0Endozoicomonas<\/i>\u00a0spp. We obtained seven novel\u00a0Endozoicomonas<\/i>\u00a0genomes and quantitative bacterial composition from\u00a0Acropora tenuis<\/i>\u00a0corals at four sites in Japan. Our quantitative 16S rRNA gene and comparative genomic analysis revealed that these\u00a0Endozoicomonas<\/i>\u00a0spp. belong to different lineages (Clade A and Clade B), with widely varying abundance among individual corals. Furthermore, each\u00a0Endozoicomonas<\/i>\u00a0species possessed various eukaryotic-like genes in clade-specific genes. It was suggested that these eukaryotic-like genes might have a potential ability of different functions in each clade, such as infection of the host coral or suppression of host immune pathways. These\u00a0Endozoicomonas<\/i>\u00a0species may have adopted different host adaptation strategies despite living symbiotically on the same coral.<\/p>\n

Conclusions<\/h3>\n

This study suggests that coral-associated\u00a0Endozoicomonas<\/i>\u00a0spp. on the same species of coral have different evolutional strategies and functional potentials in each species and emphasizes the need to analyze the genome of each uncultured strain in future coral-Endozoicomonas<\/i>\u00a0relationships studies.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Journal Title \/\u63b2\u8f09\u30b8\u30e3\u30fc\u30ca\u30eb\u540d Microbiome Publication Year and Month \/\u63b2\u8f09\u5e74\u6708 December, 2022 Paper Title \/\u8ad6\u6587\u30bf\u30a4\u30c8\u30eb Targete […]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[],"tags":[218,217],"class_list":["post-73932","post","type-post","status-publish","format-standard","hentry","tag-impact-en","tag-impact"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts\/73932","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/comments?post=73932"}],"version-history":[{"count":1,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts\/73932\/revisions"}],"predecessor-version":[{"id":73933,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts\/73932\/revisions\/73933"}],"wp:attachment":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/media?parent=73932"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/categories?post=73932"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/tags?post=73932"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}