{"id":73677,"date":"2022-11-08T13:27:18","date_gmt":"2022-11-08T04:27:18","guid":{"rendered":"https:\/\/www.waseda.jp\/inst\/research\/?p=73677"},"modified":"2022-11-09T14:34:16","modified_gmt":"2022-11-09T05:34:16","slug":"actin-binding-domain-of-rng-2-sparsely-bound-on-f-actin-strongly-inhabits-actin-movement-on-myosin%e2%85%a1%ef%bc%88published-in-life-science-aliance-october-2022%ef%bc%89","status":"publish","type":"post","link":"https:\/\/www.waseda.jp\/inst\/research\/news\/73677","title":{"rendered":"Actin binding domain of Rng-2 sparsely bound on F-actin strongly inhabits actin movement on myosin\u2161\uff08Published in Life Science Aliance, October 2022\uff09"},"content":{"rendered":"<table class=\"table table-bordered table-colored-tbhd\" style=\"height: 550px; width: 100%; border-collapse: collapse; border-style: solid;\" border=\"1\">\n<tbody>\n<tr style=\"height: 78px;\">\n<td style=\"width: 16.7893%; height: 78px;\">Journal Title<br \/>\n\/\u63b2\u8f09\u30b8\u30e3\u30fc\u30ca\u30eb\u540d<\/td>\n<td style=\"width: 71.3712%; height: 78px;\">Life Science Aliance<\/td>\n<\/tr>\n<tr style=\"height: 65px;\">\n<td style=\"width: 16.7893%; height: 80px;\">Publication Year and Month<br \/>\n\/\u63b2\u8f09\u5e74\u6708<\/td>\n<td style=\"width: 71.3712%; height: 80px;\">October, 2022<\/td>\n<\/tr>\n<tr style=\"height: 55px;\">\n<td style=\"width: 16.7893%; height: 79px;\">Paper Title<br \/>\n\/\u8ad6\u6587\u30bf\u30a4\u30c8\u30eb<\/td>\n<td style=\"width: 71.3712%; height: 79px;\">Actin binding domain of Rng-2 sparsely bound on F-actin strongly inhabits actin movement on myosin\u2161<\/td>\n<\/tr>\n<tr style=\"height: 85px;\">\n<td style=\"width: 16.7893%; height: 85px;\">DOI<br \/>\n\/\u8ad6\u6587DOI<\/td>\n<td style=\"width: 71.3712%; height: 85px;\"><a href=\"https:\/\/doi.org\/10.26508\/lsa.202201469\">10.26508\/Isa.202201469<\/a><\/td>\n<\/tr>\n<tr style=\"height: 59px;\">\n<td style=\"width: 16.7893%; height: 80px;\">\u00a0Author of Waseda University<br \/>\n\/\u672c\u5b66\u306e\u8457\u8005<\/td>\n<td style=\"width: 71.3712%; height: 80px;\">UEDA, Taro(Professor, Faculty of Science and Engineering, School of Advanced Science and Engineering):Last Author, Corresponding Author<\/td>\n<\/tr>\n<tr style=\"height: 68px;\">\n<td style=\"width: 16.7893%; height: 86px;\">Related Websites<br \/>\n\/\u95a2\u9023Web<\/td>\n<td style=\"width: 71.3712%; height: 86px;\">&#8211;<\/td>\n<\/tr>\n<tr style=\"height: 138px;\">\n<td style=\"width: 16.7893%; height: 148px;\">Abstract<br \/>\n\/\u6284\u9332<\/td>\n<td style=\"width: 71.3712%; height: 148px;\">We report a case in which sub-stoichiometric binding of an actin-binding protein has profound structural and functional consequences, providing an insight into the fundamental properties of actin regulation. Rng2 is an IQGAP contained in contractile rings in the fission yeast\u00a0<em>Schizosaccharomyces pombe<\/em>. Here, we used high-speed atomic force microscopy and electron microscopy and found that sub-stoichiometric binding of the calponin-homology actin-binding domain of Rng2 (Rng2CHD) induces global structural changes in skeletal muscle actin filaments, including shortening of the filament helical pitch. Sub-stoichiometric binding of Rng2CHD also reduced the affinity between actin filaments and muscle myosin II carrying ADP and strongly inhibited the motility of actin filaments on myosin II in vitro. On skeletal muscle myosin II\u2013coated surfaces, Rng2CHD stopped the actin movements at a binding ratio of 11%. Rng2CHD also inhibited actin movements on myosin II of the amoeba\u00a0<em>Dictyostelium<\/em>, but in this case, by detaching actin filaments from myosin II\u2013coated surfaces. Thus, sparsely bound Rng2CHD induces apparently cooperative structural changes in actin filaments and inhibits force generation by actomyosin II.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>Journal Title \/\u63b2\u8f09\u30b8\u30e3\u30fc\u30ca\u30eb\u540d Life Science Aliance Publication Year and Month \/\u63b2\u8f09\u5e74\u6708 October, 2022 Paper Title \/\u8ad6\u6587\u30bf\u30a4\u30c8 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[],"tags":[218,217],"class_list":["post-73677","post","type-post","status-publish","format-standard","hentry","tag-impact-en","tag-impact"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts\/73677","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/comments?post=73677"}],"version-history":[{"count":1,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts\/73677\/revisions"}],"predecessor-version":[{"id":73682,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts\/73677\/revisions\/73682"}],"wp:attachment":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/media?parent=73677"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/categories?post=73677"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/tags?post=73677"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}