{"id":73224,"date":"2022-08-25T12:19:44","date_gmt":"2022-08-25T03:19:44","guid":{"rendered":"https:\/\/www.waseda.jp\/inst\/research\/?p=73224"},"modified":"2022-08-31T09:41:53","modified_gmt":"2022-08-31T00:41:53","slug":"the-mis6-inner-kinetochore-subcomplex-maintains-cenp-a-nucleosomes-against-centromeric-non-cording-transcription-during-mitosis%ef%bc%88published-in-communications-biology-august-2022%ef%bc%89","status":"publish","type":"post","link":"https:\/\/www.waseda.jp\/inst\/research\/news\/73224","title":{"rendered":"The Mis6 inner kinetochore subcomplex maintains CENP-A nucleosomes against centromeric non-cording transcription during mitosis\uff08Published in Communications Biology, August 2022\uff09"},"content":{"rendered":"<table class=\"table table-bordered table-colored-tbhd\" style=\"height: 550px; width: 100%; border-collapse: collapse; border-style: solid;\" border=\"1\">\n<tbody>\n<tr style=\"height: 78px;\">\n<td style=\"width: 16.7893%; height: 78px;\">Journal Title<br \/>\n\/\u63b2\u8f09\u30b8\u30e3\u30fc\u30ca\u30eb\u540d<\/td>\n<td style=\"width: 71.3712%; height: 78px;\">Communications Biology<\/td>\n<\/tr>\n<tr style=\"height: 65px;\">\n<td style=\"width: 16.7893%; height: 80px;\">Publication Year and Month<br \/>\n\/\u63b2\u8f09\u5e74\u6708<\/td>\n<td style=\"width: 71.3712%; height: 80px;\">August, 2022<\/td>\n<\/tr>\n<tr style=\"height: 55px;\">\n<td style=\"width: 16.7893%; height: 79px;\">Paper Title<br \/>\n\/\u8ad6\u6587\u30bf\u30a4\u30c8\u30eb<\/td>\n<td style=\"width: 71.3712%; height: 79px;\">The Mis6 inner kinetochore subcomplex maintains CENP-A nucleosomes against centromeric non-cording transcription during mitosis<\/td>\n<\/tr>\n<tr style=\"height: 85px;\">\n<td style=\"width: 16.7893%; height: 85px;\">DOI<br \/>\n\/\u8ad6\u6587DOI<\/td>\n<td style=\"width: 71.3712%; height: 85px;\"><a href=\"https:\/\/doi.org\/10.1038\/s42003-022-03786-y\">10.1038\/s42003-022-03786-y<\/a><\/td>\n<\/tr>\n<tr style=\"height: 59px;\">\n<td style=\"width: 16.7893%; height: 80px;\">\u00a0Author of Waseda University<br \/>\n\/\u672c\u5b66\u306e\u8457\u8005<\/td>\n<td style=\"width: 71.3712%; height: 80px;\">SATO, Masamitsu(Professor, Faculty of Science and Engineering, School of Advanced Science and Engineering):Corresponding Author, Last Author<\/td>\n<\/tr>\n<tr style=\"height: 68px;\">\n<td style=\"width: 16.7893%; height: 86px;\">Related Websites<br \/>\n\/\u95a2\u9023Web<\/td>\n<td style=\"width: 71.3712%; height: 86px;\">&#8211;<\/td>\n<\/tr>\n<tr style=\"height: 138px;\">\n<td style=\"width: 16.7893%; height: 148px;\">Abstract<br \/>\n\/\u6284\u9332<\/td>\n<td style=\"width: 71.3712%; height: 148px;\">Centromeres are established by nucleosomes containing the histone H3 variant CENP-A. CENP-A is recruited to centromeres by the Mis18\u2013HJURP machinery. During mitosis, CENP-A recruitment ceases, implying the necessity of CENP-A maintenance at centromeres, although the exact underlying mechanism remains elusive. Herein, we show that the inner kinetochore protein Mis6 (CENP-I) and Mis15 (CENP-N) retain CENP-A during mitosis in fission yeast. Eliminating Mis6 or Mis15 during mitosis caused immediate loss of pre-existing CENP-A at centromeres. CENP-A loss occurred due to the transcriptional upregulation of non-coding RNAs at the central core region of centromeres, as confirmed by the observation RNA polymerase II inhibition preventing CENP-A loss from centromeres in the\u00a0<i>mis6<\/i>\u00a0mutant. Thus, we concluded that the inner kinetochore complex containing Mis6\u2013Mis15 blocks the indiscriminate transcription of non-coding RNAs at the core centromere, thereby retaining the epigenetic inheritance of CENP-A during mitosis.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>Journal Title \/\u63b2\u8f09\u30b8\u30e3\u30fc\u30ca\u30eb\u540d Communications Biology Publication Year and Month \/\u63b2\u8f09\u5e74\u6708 August, 2022 Paper Title \/\u8ad6\u6587\u30bf\u30a4 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[],"tags":[218,217],"class_list":["post-73224","post","type-post","status-publish","format-standard","hentry","tag-impact-en","tag-impact"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts\/73224","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/comments?post=73224"}],"version-history":[{"count":1,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts\/73224\/revisions"}],"predecessor-version":[{"id":73236,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts\/73224\/revisions\/73236"}],"wp:attachment":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/media?parent=73224"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/categories?post=73224"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/tags?post=73224"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}