{"id":51305,"date":"2017-05-23T18:08:47","date_gmt":"2017-05-23T09:08:47","guid":{"rendered":"https:\/\/www.waseda.jp\/top\/?p=51305"},"modified":"2018-10-19T15:07:47","modified_gmt":"2018-10-19T06:07:47","slug":"%e5%8c%96%e5%90%88%e7%89%a9%e3%81%ae%e6%a8%99%e7%9a%84%e5%88%86%e5%ad%90%e3%81%ae%e5%90%8c%e5%ae%9a%e3%82%92%e5%8a%b9%e7%8e%87%e5%8c%96%e3%80%80%e6%96%b0%e3%81%97%e3%81%84shrna%e3%82%b9%e3%82%af","status":"publish","type":"post","link":"https:\/\/www.waseda.jp\/inst\/research\/news\/51305","title":{"rendered":"\u5316\u5408\u7269\u306e\u6a19\u7684\u5206\u5b50\u306e\u540c\u5b9a\u3092\u52b9\u7387\u5316\u3000\u65b0\u3057\u3044shRNA\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3067\u8907\u6570\u8a66\u6599\u306e\u540c\u6642\u89e3\u6790\u3092\u53ef\u80fd\u306b"},"content":{"rendered":"<h2>\u5316\u5408\u7269\u306e\u6a19\u7684\u5206\u5b50\u306e\u540c\u5b9a\u3092\u52b9\u7387\u5316<\/h2>\n<h3>\u65b0\u3057\u3044shRNA\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3067\u8907\u6570\u8a66\u6599\u306e\u540c\u6642\u89e3\u6790\u3092\u53ef\u80fd\u306b<\/h3>\n<p><a href=\"http:\/\/www.riken.jp\/pr\/press\/2017\/20170523_2\/\" target=\"_blank\">\u7406\u5316\u5b66\u7814\u7a76\u6240\uff08\u7406\u7814\uff09<\/a>\u74b0\u5883\u8cc7\u6e90\u79d1\u5b66\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u30b1\u30df\u30ab\u30eb\u30b2\u30ce\u30df\u30af\u30b9\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306e\u9ad9\u702c\u7fd4\u5e73\u7814\u4fee\u751f\u3001\u5409\u7530\u7a14\u30b0\u30eb\u30fc\u30d7\u30c7\u30a3\u30ec\u30af\u30bf\u30fc\u3001\u677e\u672c\u5065\u5c02\u4efb\u7814\u7a76\u54e1\u3001\u65e9\u7a32\u7530\u5927\u5b66\u7406\u5de5\u5b66\u8853\u9662\u306e<a href=\"https:\/\/researchers.waseda.jp\/profile\/ja.751d3a81269f5c132c2928477c1106f0.html\" target=\"_blank\">\u65b0\u4e95\u5927\u7950\u6b21\u5e2d\u7814\u7a76\u54e1<\/a>\u3001<a href=\"https:\/\/researchers.waseda.jp\/profile\/ja.8c029c359e7d1e6dae1955bd829c2dfc.html\" target=\"_blank\">\u4e2d\u5c3e\u6d0b\u4e00\u6559\u6388<\/a>\u3089\u306e\u5171\u540c\u7814\u7a76\u30b0\u30eb\u30fc\u30d7<sup>\u203b<\/sup>\u306f\u3001\u85ac\u306e\u5143\u3068\u306a\u308b\u5316\u5408\u7269\u306e\u4f5c\u7528\u30e1\u30ab\u30cb\u30ba\u30e0\u306e\u540c\u5b9a\u306b\u5229\u7528\u3055\u308c\u308b\u300cshRNA <sup>[1]<\/sup>\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0<sup>[2]<\/sup>\u300d\u3092\u52b9\u7387\u5316\u3057\u3001\u3053\u306e\u624b\u6cd5\u3092\u7528\u3044\u3066\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9<sup>[3]<\/sup>\u3092\u8a98\u5c0e\u3059\u308b\u6d77\u6d0b\u5929\u7136\u7269\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B<sup>[4]<\/sup>\u306e\u4f5c\u7528\u30e1\u30ab\u30cb\u30ba\u30e0\u306b\u95a2\u308f\u308b\u65b0\u305f\u306a\u907a\u4f1d\u5b50\u3092\u540c\u5b9a\u3057\u307e\u3057\u305f\u3002<br \/>\n\u57f9\u990a\u7d30\u80de\u306bshRNA\u3092\u5c0e\u5165\u3059\u308b\u3053\u3068\u3067\u3001\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u3092\u30ce\u30c3\u30af\u30c0\u30a6\u30f3<sup>[5]<\/sup>\uff08\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5408\u6210\u3092\u6291\u5236\uff09\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3055\u307e\u3056\u307e\u306ashRNA\u3092\u96c6\u3081\u305f\u3082\u306e\u3092shRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3068\u547c\u3073\u307e\u3059\u304c\u3001\u57f9\u990a\u7d30\u80de\u7fa4\u306bshRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u5c0e\u5165\u3059\u308b\u3053\u3068\u3067\u3001\u3055\u307e\u3056\u307e\u306a\u907a\u4f1d\u5b50\u3092\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u3055\u305b\u305f\u57f9\u990a\u7d30\u80de\u7fa4\u3092\u4f5c\u6210\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u306eshRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u5c0e\u5165\u3057\u305f\u57f9\u990a\u7d30\u80de\u7fa4\u306b\u5bfe\u3057\u3066\u5316\u5408\u7269\u51e6\u7406\u3092\u884c\u3046\u3053\u3068\u3067\u3001\u5316\u5408\u7269\u306e\u4f5c\u7528\u30e1\u30ab\u30cb\u30ba\u30e0\u306b\u95a2\u308f\u308b\u907a\u4f1d\u5b50\u3092\u7db2\u7f85\u7684\u306b\u8abf\u3079\u308b\u3053\u3068\uff08\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\uff09\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u306eshRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u306f\u3001\u5316\u5408\u7269\u306e\u76f4\u63a5\u306e\u6a19\u7684\u5206\u5b50\u306e\u540c\u5b9a\u3060\u3051\u3067\u306a\u304f\u3001\u5316\u5408\u7269\u3078\u306e\u611f\u53d7\u6027\u306b\u95a2\u308f\u308b\u907a\u4f1d\u5b50\u7fa4\u3092\u540c\u5b9a\u3067\u304d\u308b\u70b9\u3067\u512a\u308c\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u3053\u308c\u307e\u3067\u3001\u7d30\u80de\u3067\u306e\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u5f8c\u306eshRNA\u540c\u5b9a\u306e\u6bb5\u968e\u3067\u3001\u30b5\u30f3\u30d7\u30eb\u3054\u3068\u306b\u6b21\u4e16\u4ee3\u30b7\u30fc\u30af\u30a8\u30f3\u30b5\u30fc<sup>[6]<\/sup>\u306b\u3088\u308b\u89e3\u6790\u3092\u884c\u3063\u3066\u3044\u305f\u305f\u3081\u3001\u51e6\u7406\u52b9\u7387\u304c\u4f4e\u304f\u30b3\u30b9\u30c8\u304c\u9ad8\u3044\u3068\u3044\u3046\u8ab2\u984c\u304c\u3042\u308a\u307e\u3057\u305f\u3002<br \/>\n\u4eca\u56de\u3001\u5171\u540c\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u30b2\u30ce\u30e0\u30ef\u30a4\u30c9\u306ashRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u306b\u304a\u3044\u3066\u3001\u6b21\u4e16\u4ee3\u30b7\u30fc\u30af\u30a8\u30f3\u30b5\u30fc\u3067\u4e00\u5ea6\u306b\u8907\u6570\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u89e3\u6790\u3067\u304d\u308b\u65b9\u6cd5\u3092\u78ba\u7acb\u3057\u3001\u518d\u73fe\u6027\u3084\u5b9a\u91cf\u6027\u306b\u3064\u3044\u3066\u78ba\u8a8d\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u51e6\u7406\u52b9\u7387\u306e\u5411\u4e0a\u3068\u4f4e\u30b3\u30b9\u30c8\u5316\uff08\u5f93\u6765\u306e\u6570\u5206\u306e1\uff09\u304c\u5b9f\u73fe\u3055\u308c\u307e\u3057\u305f\u3002\u6b21\u306b\u3001\u3053\u306e\u65b9\u6cd5\u3092\u7528\u3044\u3066\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u3092\u8a98\u5c0e\u3059\u308b\u6d77\u6d0b\u5929\u7136\u7269\u306e\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u306e\u4f5c\u7528\u30e1\u30ab\u30cb\u30ba\u30e0\u306b\u95a2\u308f\u308b\u907a\u4f1d\u5b50\u306e\u540c\u5b9a\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u3078\u306e\u611f\u53d7\u6027\u306b\u95a2\u308f\u308b\u907a\u4f1d\u5b50\u3068\u3057\u3066<em>ATP1A1<\/em>\u907a\u4f1d\u5b50\u3092\u540c\u5b9a\u3057\u307e\u3057\u305f\u3002<em>ATP1A1<\/em>\u907a\u4f1d\u5b50\u306f\u3001\u7d30\u80de\u819c\u306eNa<sup>\uff0b<\/sup>\/K<sup>\uff0b<\/sup>ATP\u30a2\u30fc\u30bc<sup>[7]<\/sup>\uff08\u30ca\u30c8\u30ea\u30a6\u30e0\u30dd\u30f3\u30d7\uff09\u306e\u03b1\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u306e\u907a\u4f1d\u5b50\u3067\u3059\u3002\u307e\u305f\u3001ATP1A1\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u963b\u5bb3\u5264\u3067\u3042\u308b\u5f37\u5fc3\u914d\u7cd6\u4f53<sup>[8]<\/sup>\u306e\u30a6\u30a2\u30d0\u30a4\u30f3<sup>[9]<\/sup>\u3092\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u3068\u5171\u306b\u7d30\u80de\u306b\u4f5c\u7528\u3055\u305b\u305f\u3068\u3053\u308d\u3001\u5358\u72ec\u306e\u5834\u5408\u3088\u308a\u3082\u4f4e\u6fc3\u5ea6\u3067\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u304c\u7d30\u80de\u5897\u6b96\u6291\u5236\u3092\u5f15\u304d\u8d77\u3053\u3059\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002<br \/>\n\u4eca\u56de\u78ba\u7acb\u3057\u305f\u624b\u6cd5\u306b\u3088\u308a\u3001\u4eca\u5f8c\u3001\u591a\u304f\u306e\u5316\u5408\u7269\u306e\u4f5c\u7528\u30e1\u30ab\u30cb\u30ba\u30e0\u306b\u95a2\u308f\u308b\u907a\u4f1d\u5b50\u3092\u660e\u3089\u304b\u306b\u3067\u304d\u308b\u3068\u671f\u5f85\u3067\u304d\u307e\u3059\u3002<br \/>\n\u672c\u7814\u7a76\u306f\u3001\u82f1\u56fd\u306e\u30aa\u30f3\u30e9\u30a4\u30f3\u79d1\u5b66\u96d1\u8a8c\u300e<a href=\"http:\/\/dx.doi.org\/10.1038\/s41598-017-02016-4\" target=\"_blank\"><em>Scientific Reports<\/em><\/a>\u300f\uff085\u670817\u65e5\u4ed8\u3051\uff1a\u65e5\u672c\u6642\u95935\u670817\u65e5\uff09\u306b\u63b2\u8f09\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n<h6>\u203b\u5171\u540c\u7814\u7a76\u30b0\u30eb\u30fc\u30d7<\/h6>\n<ul>\n<li>\u7406\u5316\u5b66\u7814\u7a76\u6240\u3000\u74b0\u5883\u8cc7\u6e90\u79d1\u5b66\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u3000\u30b1\u30df\u30ab\u30eb\u30b2\u30ce\u30df\u30af\u30b9\u7814\u7a76\u30b0\u30eb\u30fc\u30d7<br \/>\n\u7814\u4fee\u751f\u3000\u9ad9\u702c\u3000\u7fd4\u5e73\u3000\uff08\u305f\u304b\u305b\u3000\u3057\u3087\u3046\u3078\u3044\uff09<br \/>\n\u30b0\u30eb\u30fc\u30d7\u30c7\u30a3\u30ec\u30af\u30bf\u30fc \u3000 \u5409\u7530\u3000\u7a14\u3000\u3000\uff08\u3088\u3057\u3060\u3000\u307f\u306e\u308b\uff09<br \/>\n\u5c02\u4efb\u7814\u7a76\u54e1\u3000 \u677e\u672c\u3000\u5065\u3000\u3000\uff08\u307e\u3064\u3082\u3068\u3000\u3051\u3093\uff09<br \/>\n\u30c6\u30af\u30cb\u30ab\u30eb\u30b9\u30bf\u30c3\u30d5\u2160\u3000\u9ed2\u5ddd\u3000\u7559\u7f8e\u3000\uff08\u304f\u308d\u304b\u308f\u3000\u308b\u307f\uff09<\/li>\n<\/ul>\n<ul>\n<li>\u660e\u6cbb\u5927\u5b66\u5927\u5b66\u9662\u3000\u8fb2\u5b66\u7814\u7a76\u79d1<br \/>\n\u51c6\u6559\u6388\u3000\u4e45\u57ce\u3000\u54f2\u592b\u3000\uff08\u304f\u3057\u308d\u3000\u3066\u3064\u304a\uff09<\/li>\n<\/ul>\n<ul>\n<li>\u65e9\u7a32\u7530\u5927\u5b66\u7406\u5de5\u5b66\u8853\u9662<br \/>\n\u6559\u6388\u3000\u4e2d\u5c3e\u3000\u6d0b\u4e00\u3000\uff08\u306a\u304b\u304a\u3000\u3088\u3046\u3044\u3061\uff09<br \/>\n\u6b21\u5e2d\u7814\u7a76\u54e1\u3000\u65b0\u4e95\u3000\u5927\u7950\u3000\uff08\u3042\u3089\u3044\u3000\u3060\u3044\u3059\u3051\uff09<\/li>\n<\/ul>\n<ul>\n<li>\u5317\u6d77\u9053\u5927\u5b66\u5927\u5b66\u9662\u3000\u5730\u7403\u74b0\u5883\u79d1\u5b66\u7814\u7a76\u9662<br \/>\n\u6559\u6388\u3000\u6c96\u91ce\u3000\u9f8d\u6587\u3000\uff08\u304a\u304d\u306e\u3000\u305f\u3064\u3075\u307f\uff09<\/li>\n<\/ul>\n<ul>\n<li>\u30df\u30af\u30ed\u30cd\u30b7\u30a2\u9023\u90a6\u30c1\u30e5\u30fc\u30af\u5dde\u30b3\u30df\u30e5\u30cb\u30c6\u30a3 \u30ab\u30ec\u30c3\u30b8<br \/>\nCampus Dean\u3000 \u30ab\u30a4\u30f3\u30c9\u30fb\u30ab\u30cd\u30e2\u30c8\u30fb\u30ab\u30f3\u30c8\uff08Kind Kanemoto Kanto\uff09<\/li>\n<\/ul>\n<h4>\uff11\uff0e\u80cc\u666f<\/h4>\n<p>\u57f9\u990a\u7d30\u80de\u306bshRNA\u3092\u5c0e\u5165\u3059\u308b\u3053\u3068\u3067\u3001\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u3092\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\uff08\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5408\u6210\u3092\u6291\u5236\uff09\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3055\u307e\u3056\u307e\u306ashRNA\u3092\u96c6\u3081\u305f\u3082\u306e\u3092shRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3068\u547c\u3073\u307e\u3059\u304c\u3001\u57f9\u990a\u7d30\u80de\u7fa4\u306bshRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u5c0e\u5165\u3059\u308b\u3053\u3068\u3067\u3001\u3055\u307e\u3056\u307e\u306a\u907a\u4f1d\u5b50\u3092\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u3057\u305f\u57f9\u990a\u7d30\u80de\u7fa4\u3092\u4f5c\u6210\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u306eshRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u5c0e\u5165\u3057\u305f\u57f9\u990a\u7d30\u80de\u7fa4\u306b\u5bfe\u3057\u3066\u5316\u5408\u7269\u51e6\u7406\u3092\u884c\u3046\u3053\u3068\u3067\u3001\u5316\u5408\u7269\u306e\u4f5c\u7528\u30e1\u30ab\u30cb\u30ba\u30e0\u306b\u95a2\u308f\u308b\u907a\u4f1d\u5b50\u3092\u7db2\u7f85\u7684\u306b\u8abf\u3079\u308b\u3053\u3068\uff08\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\uff09\u304c\u3067\u304d\u307e\u3059\uff08\u56f31\uff09\u3002<br \/>\n\u4f8b\u3048\u3070\u3001shRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u5c0e\u5165\u3057\u305f\u57f9\u990a\u7d30\u80de\u7fa4\u306b\u3001\u7d30\u80de\u5897\u6b96\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u5316\u5408\u7269\u3092\u51e6\u7406\u3057\u3001\u751f\u304d\u6b8b\u3063\u305f\u7d30\u80de\u304c\u3069\u306eshRNA\u3092\u767a\u73fe\u3057\u3066\u3044\u305f\u304b\u3092\u975e\u51e6\u7406\u7d30\u80de\uff08\u5bfe\u7167\uff09\u3068\u6bd4\u8f03\u3057\u307e\u3059\u3002\u8a72\u5f53\u3059\u308bshRNA\u3092\u540c\u5b9a\u3059\u308b\u3053\u3068\u3067\u3001\u7d30\u80de\u5897\u6b96\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u5316\u5408\u7269\u306e\u4f5c\u7528\u30e1\u30ab\u30cb\u30ba\u30e0\u306b\u95a2\u308f\u308b\u907a\u4f1d\u5b50\u3060\u3051\u3067\u306a\u304f\u3001\u5316\u5408\u7269\u3068\u5408\u6210\u81f4\u6b7b\u6027<sup>[10]<\/sup>\u3092\u793a\u3059\u907a\u4f1d\u5b50\u306e\u60c5\u5831\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u3053\u308c\u307e\u3067\u5171\u540c\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u304c\u7528\u3044\u3066\u3044\u308bshRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3067\u306f\u3001shRNA\u306e\u540c\u5b9a\u306e\u969b\u3001\u30b5\u30f3\u30d7\u30eb\u3054\u3068\u306b\u6b21\u4e16\u4ee3\u30b7\u30fc\u30af\u30a8\u30f3\u30b5\u30fc\u306b\u3088\u308b\u89e3\u6790\u3092\u884c\u3063\u3066\u3044\u305f\u305f\u3081\u3001\u51e6\u7406\u52b9\u7387\u304c\u4f4e\u304f\u30b3\u30b9\u30c8\u304c\u9ad8\u3044\u3068\u3044\u3046\u8ab2\u984c\u304c\u3042\u308a\u307e\u3057\u305f\u3002<br \/>\n\u305d\u3053\u3067\u3001\u5171\u540c\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001shRNA\u306e\u540c\u5b9a\u306b\u8907\u6570\u30b5\u30f3\u30d7\u30eb\u3092\u540c\u6642\u306b\u89e3\u6790\u3067\u304d\u308b\u624b\u6cd5\u306e\u958b\u767a\u3092\u8a66\u307f\u307e\u3057\u305f\u3002<br \/>\n<div id=\"attachment_51315\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-51315 size-medium\" src=\"https:\/\/www.waseda.jp\/top\/assets\/uploads\/2017\/05\/20170523_fig1-610x413.png\" alt=\"20170523_fig1\" width=\"610\" height=\"413\" \/><p class=\"wp-caption-text\">\u56f31 shRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u306e\u6982\u8981\u56f3 \u3055\u307e\u3056\u307e\u306ashRNA\u3092\u96c6\u3081\u305fshRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u57f9\u990a\u7d30\u80de\u7fa4\u306b\u5c0e\u5165\u3059\u308b\u3053\u3068\u3067\u3001\u3055\u307e\u3056\u307e\u306a\u907a\u4f1d\u5b50\u3092\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u3057\u305f\u57f9\u990a\u7d30\u80de\u7fa4\u3092\u4f5c\u6210\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3002shRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u5c0e\u5165\u3057\u305f\u5f8c\u306b\u5316\u5408\u7269\u3067\u51e6\u7406\u3057\u305f\u7d30\u80de\u7fa4\u3068\u975e\u51e6\u7406\u7fa4\u306eshRNA\u306e\u767a\u73fe\u72b6\u6cc1\u3092\u6bd4\u8f03\u3059\u308b\u3053\u3068\u3067\u3001\u5316\u5408\u7269\u306e\u4f5c\u7528\u30e1\u30ab\u30cb\u30ba\u30e0\u306b\u95a2\u308f\u308b\u907a\u4f1d\u5b50\u3092\u7db2\u7f85\u7684\u306b\u8abf\u3079\u308b\u3053\u3068\uff08\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\uff09\u304c\u3067\u304d\u308b\u3002<\/p><\/div><\/p>\n<h4>\uff12\uff0e\u7814\u7a76\u624b\u6cd5\u3068\u6210\u679c<\/h4>\n<p>\u5171\u540c\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u307e\u305a\u3001\u6b21\u4e16\u4ee3\u30b7\u30fc\u30af\u30a8\u30f3\u30b5\u30fc\u306b\u3088\u308b\u89e3\u6790\u306e\u524d\u306b\u3001\u7570\u306a\u308b\u30b5\u30f3\u30d7\u30eb\u540c\u58eb\u3092\u533a\u5225\u3067\u304d\u308b\u3088\u3046\u30018\u7a2e\u985e\u306e\u30a4\u30f3\u30c7\u30c3\u30af\u30b9\u914d\u5217\uff088\u5869\u57fa\u914d\u5217\uff09\u3092\u6301\u3064\u30d7\u30e9\u30a4\u30de\u30fc<sup>[11]<\/sup>\u3092\u7528\u3044\u3066shRNA\u306e\u691c\u51fa\u7528\u7279\u7570\u7684\u914d\u5217\u3067\u3042\u308b\u30d0\u30fc\u30b3\u30fc\u30c9\u90e8\u5206\uff0818\u5869\u57fa\u914d\u5217\uff09\u3092\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u9023\u9396\u53cd\u5fdc\uff08PCR\uff09<sup>[11]<\/sup>\u3067\u5897\u5e45\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u3044\u304f\u3064\u304b\u306e\u30b5\u30f3\u30d7\u30eb\u306ePCR\u7523\u7269\u3092\u6df7\u5408\u3057\u3066\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u3092\u884c\u3063\u305f\u5f8c\u306b\u3001\u30a4\u30f3\u30c7\u30c3\u30af\u30b9\u914d\u5217\u306b\u3088\u3063\u3066\u30b5\u30f3\u30d7\u30eb\u3092\u5206\u985e\u3057\u3001\u30b5\u30f3\u30d7\u30eb\u5185\u3067\u306eshRNA\u306e\u5897\u6e1b\u3092\u8abf\u3079\u308b\u3053\u3068\u304c\u53ef\u80fd\u306b\u306a\u308a\u307e\u3057\u305f\uff08\u30a4\u30f3\u30c7\u30c3\u30af\u30b9\u4ed8\u304d\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u6cd5\u3001\u56f32\uff09\u3002<br \/>\n<div id=\"attachment_51316\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-51316 size-medium\" src=\"https:\/\/www.waseda.jp\/top\/assets\/uploads\/2017\/05\/20170523_fig2-610x304.png\" alt=\"20170523_fig2\" width=\"610\" height=\"304\" \/><p class=\"wp-caption-text\">\u56f32 \u30a4\u30f3\u30c7\u30c3\u30af\u30b9\u4ed8\u304d\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u6cd5 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\/>\n\u6b21\u306b\u3001\u540c\u4e00\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u3053\u306e\u30a4\u30f3\u30c7\u30c3\u30af\u30b9\u4ed8\u304d\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u6cd5\u3068\u5f93\u6765\u6cd5\u3067\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u3092\u884c\u3044\u6bd4\u8f03\u3057\u305f\u3068\u3053\u308d\u3001\u30a4\u30f3\u30c7\u30c3\u30af\u30b9\u3092\u4ed8\u3051\u3066\u3082\u5f93\u6765\u6cd5\u306b\u6bd4\u3079\u3066\u7d50\u679c\u306b\u5f71\u97ff\u304c\u306a\u3044\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3001\u540c\u4e00\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u7570\u306a\u308b\u30a4\u30f3\u30c7\u30c3\u30af\u30b9\u914d\u5217\u3092\u6301\u30642\u7a2e\u985e\u306ePCR\u30d7\u30e9\u30a4\u30de\u30fc\u3067\u5897\u5e45\u3057\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u3092\u884c\u3063\u3066\u3082\u3001\u975e\u5e38\u306b\u518d\u73fe\u6027\u306e\u9ad8\u3044\u7d50\u679c\u304c\u5f97\u3089\u308c\u307e\u3057\u305f\uff08\u56f33\uff09\u3002<br \/>\n<div id=\"attachment_51317\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-51317 size-full\" src=\"https:\/\/www.waseda.jp\/top\/assets\/uploads\/2017\/05\/20170523_fig3.png\" alt=\"20170523_fig3\" width=\"333\" height=\"341\" \/><p class=\"wp-caption-text\">\u56f33 \u30a4\u30f3\u30c7\u30c3\u30af\u30b9\u4ed8\u304d\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u6cd5\u306e\u518d\u73fe\u6027 \u540c\u3058\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u30012\u7a2e\u985e\u306e\u30a4\u30f3\u30c7\u30c3\u30af\u30b9\u4ed8\u304d\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u7528\u3044\u3066PCR\u3092\u884c\u3044\u3001\u305d\u308c\u305e\u308c\u306e\u30d0\u30fc\u30b3\u30fc\u30c9\u306e\u30ea\u30fc\u30c9\u6570\u3092\u6bd4\u8f03\u3057\u305f\u30022\u7a2e\u985e\u306e\u30a4\u30f3\u30c7\u30c3\u30af\u30b9\u9593\uff08X\u8ef8\uff1a\u30a4\u30f3\u30c7\u30c3\u30af\u30b9a\u3001Y\u8ef8\uff1a\u30a4\u30f3\u30c7\u30c3\u30af\u30b9b\uff09\u3067\u975e\u5e38\u306b\u518d\u73fe\u6027\u306e\u9ad8\u3044\u7d50\u679c\u304c\u5f97\u3089\u308c\u305f\u3002<\/p><\/div><br \/>\n\u307e\u305f\u3001\u672c\u6765\u3001shRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u4e2d\u306b\u306a\u3044\u30d0\u30fc\u30b3\u30fc\u30c9\u914d\u5217\u3092\u6dfb\u52a0\u3057\u3066\u30a4\u30f3\u30c7\u30c3\u30af\u30b9\u4ed8\u304d\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u6cd5\u3067\u306e\u89e3\u6790\u3092\u884c\u3063\u305f\u3068\u3053\u308d\u3001\u6dfb\u52a0\u91cf\u306b\u4f34\u3063\u3066\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u306e\u30ea\u30fc\u30c9\u6570\u304c\u5897\u52a0\u3057\u305f\u3053\u3068\u304b\u3089\u3001\u672c\u6cd5\u304c\u5b9a\u91cf\u6027\u3088\u304fshRNA\u91cf\u3092\u6c7a\u5b9a\u3067\u304d\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u89e3\u6790\u30b3\u30b9\u30c8\u3082\u5f93\u6765\u306e\u6570\u5206\u306e1\u306b\u306a\u308a\u307e\u3057\u305f\u3002<br \/>\n\u305d\u3053\u3067\u5b9f\u969b\u306b\u3001\u3053\u306eshRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3067\u5316\u5408\u7269\u306e\u6a19\u7684\u5206\u5b50\u304c\u540c\u5b9a\u3067\u304d\u308b\u304b\u3069\u3046\u304b\u3092\u3001DNA\u30c8\u30dd\u30a4\u30bd\u30e1\u30e9\u30fc\u30bc\u2161<sup>[12]<\/sup>\u306e\u963b\u5bb3\u5264\u3067\u6297\u304c\u3093\u5264\u3068\u3057\u3066\u4f7f\u7528\u3055\u308c\u308b\u30a8\u30c8\u30dd\u30b7\u30c9\u3092\u7528\u3044\u3066\u8abf\u3079\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001\u30a8\u30c8\u30dd\u30b7\u30c9\u51e6\u7406\u7d30\u80de\u4e2d\u306eDNA\u30c8\u30dd\u30a4\u30bd\u30e1\u30e9\u30fc\u30bc\u2161\u306emRNA\u306b\u5bfe\u5fdc\u3059\u308bshRNA\u304c\u975e\u51e6\u7406\u7d30\u80de\u306b\u6bd4\u3079\u3066\u8457\u3057\u304f\u5897\u52a0\u3057\u305f\u3053\u3068\u304b\u3089\u3001\u672c\u6cd5\u306b\u3088\u3063\u3066\u5316\u5408\u7269\u306e\u6a19\u7684\u5206\u5b50\u306e\u540c\u5b9a\u304c\u53ef\u80fd\u3067\u3042\u308b\u3053\u3068\u304c\u78ba\u304b\u3081\u3089\u308c\u307e\u3057\u305f\u3002<br \/>\n\u6b21\u306b\u3001\u30e9\u30f3\u85fb\u304b\u3089\u5358\u96e2\u3055\u308c\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\uff08\u30d7\u30ed\u30b0\u30e9\u30e0\u3055\u308c\u305f\u7d30\u80de\u6b7b\uff09\u8a98\u5c0e\u6d3b\u6027\u3092\u6301\u3064\u3001\u6d77\u6d0b\u5929\u7136\u7269\u306e\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u306b\u3064\u3044\u3066\u3001shRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u975e\u51e6\u7406\u7d30\u80de\u306b\u6bd4\u3079\u3066\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u51e6\u7406\u7d30\u80de\u3067\u6e1b\u5c11\u3059\u308bshRNA\u306b\u6ce8\u76ee\u3057\u3066\u89e3\u6790\u3092\u9032\u3081\u3001\u5019\u88dc\u907a\u4f1d\u5b50\u306b\u5bfe\u3059\u308bsiRNA<sup>[1]<\/sup>\u3067\u4e8c\u6b21\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001\u300c<em>ATP1A1<\/em>\u907a\u4f1d\u5b50\u300d\u306e\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u306b\u3088\u3063\u3066\u3001\u7d30\u80de\u306e\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u306b\u5bfe\u3059\u308b\u611f\u53d7\u6027\u304c\u975e\u5e38\u306b\u9ad8\u304f\u306a\u308b\u3001\u3059\u306a\u308f\u3061\u4f4e\u6fc3\u5ea6\u306e\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u3067\u7d30\u80de\u5897\u6b96\u6291\u5236\u304c\u5f15\u304d\u8d77\u3053\u3055\u308c\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\uff08\u56f34\uff09\u3002<br \/>\n<em>ATP1A1<\/em>\u306f\u3001\u7d30\u80de\u819c\u306eNa<sup>\uff0b<\/sup>\/K<sup>\uff0b<\/sup>ATP\u30a2\u30fc\u30bc\uff08\u30ca\u30c8\u30ea\u30a6\u30e0\u30dd\u30f3\u30d7\uff09\u306e\u9175\u7d20\u6d3b\u6027\u3092\u62c5\u3046\u03b1\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u306e\u907a\u4f1d\u5b50\u3067\u3059\u3002Na<sup>\uff0b<\/sup>\/K<sup>\uff0b<\/sup>ATP\u30a2\u30fc\u30bc\u306e\u03b1\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u306f\u3001\u5f37\u5fc3\u4f5c\u7528\u3092\u6301\u3064\u5f37\u5fc3\u914d\u7cd6\u4f53\u306b\u3088\u3063\u3066\u963b\u5bb3\u3055\u308c\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u3053\u3067\u3001\u5f37\u5fc3\u914d\u7cd6\u4f53\u306e\u4e00\u3064\u3067\u3042\u308b\u30a6\u30a2\u30d0\u30a4\u30f3\u3068\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u3067\u7d30\u80de\u3092\u5171\u51e6\u7406\u3057\u305f\u3068\u3053\u308d\u3001\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u5358\u72ec\u306e\u5834\u5408\u3088\u308a\u3082\u4f4e\u6fc3\u5ea6\u3067\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u304c\u7d30\u80de\u5897\u6b96\u6291\u5236\u3092\u5f15\u304d\u8d77\u3053\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u30a6\u30a2\u30d0\u30a4\u30f3\u5b58\u5728\u4e0b\u3067\u306f\u3001\u7d30\u80de\u306e\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u306b\u5bfe\u3059\u308b\u611f\u53d7\u6027\u304c\u9ad8\u307e\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\uff08\u56f34\uff09\u3002<br \/>\n\u3055\u3089\u306b\u3001\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u306b\u3088\u308b\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u8a98\u5c0e\u306e\u6307\u6a19\u3067\u3042\u308bPARP\uff08\u30dd\u30ea\uff08ADP\u30ea\u30dc\u30fc\u30b9\uff09\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc1\uff09<sup>[13]<\/sup>\u306e\u5207\u65ad\u304c\u3001\u30a6\u30a2\u30d0\u30a4\u30f3\u3068\u306e\u5171\u51e6\u7406\u306b\u3088\u3063\u3066\u4fc3\u9032\u3055\u308c\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u4ee5\u4e0a\u306e\u7d50\u679c\u306f\u3001ATP1A1\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u7d30\u80de\u306e\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u306b\u5bfe\u3059\u308b\u611f\u53d7\u6027\u3092\u6c7a\u3081\u308b\u56e0\u5b50\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002<br \/>\n<div id=\"attachment_51313\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-51313 size-medium\" src=\"https:\/\/www.waseda.jp\/top\/assets\/uploads\/2017\/05\/20170523_fig4-610x443.png\" alt=\"20170523_fig4\" width=\"610\" height=\"443\" \/><p class=\"wp-caption-text\">\u56f3\uff14\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u3068ATP1A1\u963b\u5bb3\u306b\u3088\u308b\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u8a98\u5c0e\u306e\u6a21\u5f0f\u56f3 ATP1A1\u306e\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u3001\u3042\u308b\u3044\u306f\u3001ATP1A1\u963b\u5bb3\u5264\u3067\u3042\u308b\u30a6\u30a2\u30d0\u30a4\u30f3\u3068\u306e\u4f75\u7528\u306b\u3088\u3063\u3066\u3001\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u306b\u3088\u308b\u7d30\u80de\u6b7b\uff08\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\uff09\u304c\u4ea2\u9032\u3059\u308b\u3002<\/p><\/div><\/p>\n<h4>\uff13\uff0e\u4eca\u5f8c\u306e\u671f\u5f85<\/h4>\n<p>\u4eca\u56de\u958b\u767a\u3057\u305f\u30a4\u30f3\u30c7\u30c3\u30af\u30b9\u4ed8\u304d\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u6cd5\u306b\u3088\u3063\u3066shRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u306e\u51e6\u7406\u52b9\u7387\u3092\u4e0a\u3052\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u305f\u3081\u3001\u30d2\u30c8\u57f9\u990a\u7d30\u80de\u3092\u4f7f\u3063\u3066\u907a\u4f1d\u5b66\u7684\u306b\u5316\u5408\u7269\u306e\u6a19\u7684\u7d4c\u8def\u3084\u5408\u6210\u81f4\u6b7b\u7d4c\u8def\u306e\u540c\u5b9a\u304c\u9032\u3080\u3068\u671f\u5f85\u3067\u304d\u307e\u3059\u3002<br \/>\nshRNA\u3084gRNA<sup>[14]<\/sup>\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3067\u306e\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u306f\u5316\u5408\u7269\u306e\u6a19\u7684\u540c\u5b9a\u4ee5\u5916\u306b\u3082\u3001\u304c\u3093\u7d30\u80de\u306e\u8106\u5f31\u6027\u306e\u8981\u56e0\u306e\u540c\u5b9a\u3084\u3001\u75be\u60a3\u30e2\u30c7\u30eb\u7d30\u80de\u306e\u30d7\u30ed\u30d5\u30a1\u30a4\u30ea\u30f3\u30b0\u3092\u901a\u3058\u305f\u65b0\u305f\u306a\u6cbb\u7642\u6a19\u7684\u5206\u5b50\u306e\u540c\u5b9a\u306a\u3069\u306b\u3082\u5229\u7528\u3067\u304d\u308b\u305f\u3081\u3001\u4eca\u56de\u306e\u65b9\u6cd5\u306f\u4eca\u5f8c\u3001\u5e45\u5e83\u304f\u5fdc\u7528\u53ef\u80fd\u306a\u6280\u8853\u3068\u3044\u3048\u307e\u3059\u3002<br \/>\n\u767d\u8840\u75c5\u3001\u814e\u81d3\u304c\u3093\u3001\u524d\u7acb\u817a\u304c\u3093\u306e\u7d30\u80de\u682a\u306b\u304a\u3044\u3066\u3001\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u306e\u611f\u53d7\u6027\u304c\u9ad8\u3044\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u4e00\u65b9\u3001\u5f37\u5fc3\u914d\u7cd6\u4f53\u3082\u3055\u307e\u3056\u307e\u306a\u304c\u3093\u7d30\u80de\u306b\u5bfe\u3057\u3066\u9078\u629e\u7684\u306b\u5897\u6b96\u6291\u5236\u6d3b\u6027\u3092\u6301\u3064\u3053\u3068\u304c\u5206\u304b\u3063\u3066\u3044\u307e\u3059\u3002\u4eca\u5f8c\u3001\u30aa\u30fc\u30ea\u30e9\u30a4\u30c9B\u3068\u5f37\u5fc3\u914d\u7cd6\u4f53\u306e\u4f75\u7528\u306b\u3088\u308a\u3001\u3069\u306e\u3088\u3046\u306a\u304c\u3093\u7d30\u80de\u306b\u5bfe\u3059\u308b\u7d30\u80de\u5897\u6b96\u6291\u5236\u52b9\u679c\u304c\u5f37\u3044\u306e\u304b\u306a\u3069\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001\u65b0\u305f\u306a\u6cbb\u7642\u6226\u7565\u306b\u3064\u306a\u304c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<h4>\uff14\uff0e\u8ad6\u6587\u60c5\u5831<\/h4>\n<ul>\n<li>\u30bf\u30a4\u30c8\u30eb\uff1a<a href=\"http:\/\/dx.doi.org\/10.1038\/s41598-017-02016-4\" target=\"_blank\">A quantitative shRNA screen identifies ATP1A1 as a gene that regulates cytotoxicity by aurilide B<\/a><\/li>\n<\/ul>\n<ul>\n<li>\u8457\u8005\u540d\uff1aShohei Takase, Rumi Kurokawa, Daisuke Arai, Kind Kanemoto Kanto, Tatsufumi Okino, Yoichi Nakao, Tetsuo Kushiro, Minoru Yoshida, and Ken Matsumoto<\/li>\n<\/ul>\n<ul>\n<li>\u96d1\u8a8c\uff1a<em>Scientific Reports<\/em><\/li>\n<\/ul>\n<ul>\n<li>DOI\uff1a10.1038\/s41598-017-02016-4<\/li>\n<\/ul>\n<h4>\uff15\uff0e\u88dc\u8db3\u8aac\u660e<\/h4>\n<ul>\n<li><strong>[1] shRNA\u3001siRNA<\/strong>\uff1ashRNA\uff08short hairpin RNA\uff09\u306f\u30d8\u30a2\u30d4\u30f3\u578b\u306e\u5c0f\u3055\u306aRNA\uff08\u304a\u3088\u305d60\u5869\u57fa\u914d\u5217\uff09\u3067\u3042\u308a\u3001\u7d30\u80de\u5185\u306eRNA\u5207\u65ad\u9175\u7d20\u3067\u5207\u65ad\u3055\u308c\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u300121\uff5e23\u5869\u57fa\u306esiRNA\uff08small interfering RNA\uff09\u3068\u306a\u308b\u3002shRNA\u306e\u914d\u5217\u3092\u9078\u3093\u3067\u7d30\u80de\u5185\u306b\u5b58\u5728\u3055\u305b\u308b\u3068\u3001siRNA\u304cRNA\u5206\u89e3\u9175\u7d20\u3092\u7279\u5b9a\u306e\u30e1\u30c3\u30bb\u30f3\u30b8\u30e3\u30fcRNA\uff08mRNA\uff09\u306b\u4f5c\u7528\u3055\u305b\u308b\u305f\u3081\u3001\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u306e\u767a\u73fe\uff08\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5408\u6210\uff09\u3092\u7279\u7570\u7684\u306b\u963b\u5bb3\u3059\u308b\u3053\u3068\uff08RNA\u5e72\u6e09\uff09\u304c\u3067\u304d\u308b\u3002<\/li>\n<li><strong>[2] shRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0<\/strong>\uff1a\u7d30\u80de\u5185\u306b\u5b58\u5728\u3059\u308b\u3055\u307e\u3056\u307e\u306amRNA\u306b\u5bfe\u5fdc\u3059\u308bshRNA\u3092\u96c6\u3081\u305f\u3082\u306e\u3092\u3001shRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3068\u547c\u3076\u3002shRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u7d30\u80de\u306b\u5c0e\u5165\u3059\u308b\u3053\u3068\u3067\u3001\u305d\u308c\u305e\u308c\u306emRNA\u304b\u3089\u5408\u6210\u3055\u308c\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6a5f\u80fd\u3092\u7db2\u7f85\u7684\u306b\u8abf\u3079\u308b\u65b9\u6cd5\u3092shRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3068\u3044\u3046\u3002shRNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306b\u306f\u3001\u3055\u307e\u3056\u307e\u306amRNA\u306b\u5bfe\u3059\u308bshRNA\u304c\u6df7\u5408\u3055\u308c\u305f\u30d7\u30fc\u30eb\u578b\u3068\u3001\u6df7\u5408\u3055\u308c\u305a\u306b\u5225\u3005\u306e\u72b6\u614b\u306b\u306a\u3063\u3066\u3044\u308b\u30a2\u30ec\u30a4\u578b\u304c\u3042\u308a\u3001\u4eca\u56de\u306e\u7814\u7a76\u3067\u306f\u30d7\u30fc\u30eb\u578b\u3092\u7528\u3044\u305f\u3002<\/li>\n<li><strong>[3] 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src=\"https:\/\/www.waseda.jp\/top\/assets\/uploads\/2017\/05\/20170523_fig5.png\" alt=\"20170523_fig5\" width=\"250\" height=\"208\" \/><\/li>\n<li><strong>[5] \u30ce\u30c3\u30af\u30c0\u30a6\u30f3<\/strong>\uff1a\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u3092\u5927\u304d\u304f\u6e1b\u8870\u3055\u305b\u308b\u304c\u3001\u5b8c\u5168\u306b\u306f\u6b20\u5931\u3055\u305b\u306a\u3044\u72b6\u614b\u306b\u3059\u308b\u3053\u3068\u3002mRNA\u306e\u5206\u89e3\u4fc3\u9032\u3084\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7ffb\u8a33\u963b\u5bb3\u306a\u3069\u3001\u907a\u4f1d\u5b50\u7834\u58ca\u4ee5\u5916\u306e\u64cd\u4f5c\u306b\u3088\u308a\u884c\u3046\u5834\u5408\u304c\u591a\u3044\u3002<\/li>\n<li><strong>[6] \u6b21\u4e16\u4ee3\u30b7\u30fc\u30af\u30a8\u30f3\u30b5\u30fc<\/strong>\uff1aDNA\u306e\u5869\u57fa\u914d\u5217\u3092\u6c7a\u5b9a\u3059\u308b\u305f\u3081\u306e\u88c5\u7f6e\u3067\u3001\u5f93\u6765\u306e\u65b9\u6cd5\u3068\u7570\u306a\u308b\u65b9\u6cd5\u3067\u3088\u308a\u9ad8\u901f\u9ad8\u7cbe\u5ea6\u306b\u5869\u57fa\u914d\u5217\u3092\u6c7a\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3002\u30d2\u30c8\u30b2\u30ce\u30e0\u89e3\u8aad\u3092\u76ee\u6307\u3057\u3066\u958b\u767a\u3055\u308c\u305f\u3002<\/li>\n<li><strong>[7] Na<sup>\uff0b<\/sup>\/K<sup>\uff0b<\/sup>ATP\u30a2\u30fc\u30bc<\/strong>\uff1a\u7d30\u80de\u5185\u306f\u7d30\u80de\u5916\u306b\u6bd4\u3079\u308b\u3068\u30ca\u30c8\u30ea\u30a6\u30e0\u30a4\u30aa\u30f3\uff08Na<sup>\uff0b<\/sup>\uff09\u6fc3\u5ea6\u304c\u4f4e\u304f\u3001\u30ab\u30ea\u30a6\u30e0\u30a4\u30aa\u30f3\uff08K<sup>\uff0b<\/sup>\uff09\u304c\u9ad8\u3044\u3002\u3053\u306e\u72b6\u614b\u3092\u4fdd\u3064\u305f\u3081\u306b\u3001\u30ca\u30c8\u30ea\u30a6\u30e0\u30a4\u30aa\u30f3\uff08Na<sup>\uff0b<\/sup>\uff09\u3092\u7d30\u80de\u5916\u3078\u3001\u30ab\u30ea\u30a6\u30e0\u30a4\u30aa\u30f3\uff08K<sup>\uff0b<\/sup>\uff09\u3092\u7d30\u80de\u5185\u3078\u3001ATP\u3092\u6d88\u8cbb\u3057\u3066\u8f38\u9001\u3059\u308b\u30dd\u30f3\u30d7\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u304cNa<sup>\uff0b<\/sup>\/K<sup>\uff0b<\/sup>ATP\u30a2\u30fc\u30bc\u3067\u3042\u308b\u3002\u30ca\u30c8\u30ea\u30a6\u30e0\u30dd\u30f3\u30d7\u3068\u3082\u547c\u3070\u308c\u3001\u03b1\u3068\u03b2\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u3001\u88dc\u8db3\u7684\u306a\u03b3\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u304b\u3089\u306a\u308b\u3002<\/li>\n<li><strong>[8] \u5f37\u5fc3\u914d\u7cd6\u4f53<\/strong>\uff1a\u5f37\u5fc3\u4f5c\u7528\uff08\u5fc3\u81d3\u306e\u53ce\u7e2e\u529b\u3092\u5f37\u304f\u3059\u308b\uff09\u3092\u6301\u3064\u30b9\u30c6\u30ed\u30a4\u30c9\u914d\u7cd6\u4f53\u306e\u3053\u3068\u3067\u3001\u5fc3\u4e0d\u5168\u3084\u4e0d\u6574\u8108\u7528\u306e\u85ac\u3068\u3057\u3066\u7528\u3044\u3089\u308c\u308b\u3002Na<sup>\uff0b<\/sup>\/K<sup>\uff0b<\/sup>ATP\u30a2\u30fc\u30bc\u306b\u7279\u7570\u7684\u306b\u7d50\u5408\u3057\u3066\u6d3b\u6027\u3092\u963b\u5bb3\u3059\u308b\u3002<\/li>\n<li><strong>[9] \u30a6\u30a2\u30d0\u30a4\u30f3<\/strong>\uff1a\u5f37\u5fc3\u914d\u7cd6\u4f53\u306e\u4e00\u3064\u3067\u3001\u30ea\u30f3\u30c9\u30a6\u76ee\u30ad\u30e7\u30a6\u30c1\u30af\u30c8\u30a6\u79d1\u306e\u690d\u7269<em>Strophanthus gratus<\/em>\u306a\u3069\u306e\u7a2e\u5b50\u306b\u542b\u307e\u308c\u308b\u3002\u30a2\u30d5\u30ea\u30ab\u306e\u30bd\u30de\u30ea\u65cf\u306e\u9593\u3067\u3001\u6bd2\u77e2\u306e\u6bd2\u3068\u3057\u3066\u7528\u3044\u3089\u308c\u3066\u3044\u305f\u3002<\/li>\n<li><strong>[10] \u5408\u6210\u81f4\u6b7b\u6027<\/strong>\uff1a\u5358\u72ec\u306e\u5909\u7570\u3067\u306f\u7d30\u80de\u304c\u6b7b\u306b\u81f3\u3089\u306a\u3044\u8907\u6570\u306e\u907a\u4f1d\u5b50\u304c\u540c\u6642\u306b\u5909\u7570\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001\u7d30\u80de\u304c\u6b7b\u306b\u81f3\u308b\u95a2\u4fc2\u6027\u306e\u3053\u3068\u3002<\/li>\n<li><strong>[11] \u30d7\u30e9\u30a4\u30de\u30fc\u3001\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u9023\u9396\u53cd\u5fdc\uff08PCR\uff09<\/strong>\uff1a\u5fae\u91cfDNA\u3092\u9175\u7d20\u53cd\u5fdc\u3067\u5897\u5e45\u3059\u308b\u53cd\u5fdc\u3092PCR\u3068\u547c\u3076\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u306fPCR\u306e\u969b\u306b\u7528\u3044\u3089\u308c\u308b2\u7a2e\u985e\u306e\u30aa\u30ea\u30b4\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u3002<\/li>\n<li><strong>[12] DNA\u30c8\u30dd\u30a4\u30bd\u30e1\u30e9\u30fc\u30bc\u2161<\/strong>\uff1a\u4e8c\u672c\u9396DNA\u306f\u4e8c\u91cd\u3089\u305b\u3093\u69cb\u9020\u3092\u5f62\u6210\u3057\u3066\u3044\u308b\u3002\u8ee2\u5199\u3001\u8907\u88fd\u3001\u4fee\u5fa9\u306e\u969b\u306b\u306f\u4e8c\u91cd\u3089\u305b\u3093\u69cb\u9020\u3092\u307b\u3069\u304f\u5fc5\u8981\u304c\u3042\u308b\u304c\u3001\u305d\u306e\u969b\u306b\u554f\u984c\u306b\u306a\u308b\u306d\u3058\u308c\u3092\u8abf\u7bc0\u3057\u3066\u3044\u308b\u306e\u304c\u30c8\u30dd\u30a4\u30bd\u30e1\u30e9\u30fc\u30bc\u3067\u3042\u308b\u3002DNA\u306e1\u672c\u3060\u3051\u3092\u5207\u65ad\u3059\u308b\u9175\u7d20\u3092\u30c8\u30dd\u30a4\u30bd\u30e1\u30e9\u30fc\u30bc\u2160\u30012\u672c\u3068\u3082\u5207\u65ad\u3059\u308b\u9175\u7d20\u3092\u30c8\u30dd\u30a4\u30bd\u30e1\u30e9\u30fc\u30bc\u2161\u3068\u3044\u3046\u3002<\/li>\n<li><strong>[13] PARP\uff08\u30dd\u30ea\uff08ADP\u30ea\u30dc\u30fc\u30b9\uff09\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc1\uff09<\/strong>\uff1a\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u30dd\u30eaADP\u30ea\u30dc\u30b7\u30eb\u5316\u3059\u308b\u9175\u7d20\u3002\u7d30\u80de\u306e\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u306e\u969b\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u9175\u7d20\u306b\u3088\u3063\u3066\u5207\u65ad\u3055\u308c\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u304a\u308a\u3001\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u304c\u8d77\u304d\u3066\u3044\u308b\u7d30\u80de\u306e\u6307\u6a19\u3068\u3057\u3066\u7528\u3044\u3089\u308c\u308b\u3002<\/li>\n<li><strong>[14] gRNA<\/strong>\uff1agRNA\uff08guide RNA\uff09\u306f\u5c0f\u3055\u3044RNA\u3067\u3001\u30b2\u30ce\u30e0DNA\u306e\u7279\u5b9a\u306e\u90e8\u5206\u306b\u76f8\u88dc\u7684\u306a\u914d\u5217\u3092\u542b\u3080\u3002CRISPR-Cas\u30b7\u30b9\u30c6\u30e0\u306b\u3088\u308b\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u304a\u3044\u3066\u3001Cas9\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u3092\u30b2\u30ce\u30e0DNA\u306e\u7279\u5b9a\u306e\u90e8\u5206\u306b\u4f5c\u7528\u3055\u305b\u308b\u305f\u3081\u306b\u7528\u3044\u3089\u308c\u308b\u3002<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u5316\u5408\u7269\u306e\u6a19\u7684\u5206\u5b50\u306e\u540c\u5b9a\u3092\u52b9\u7387\u5316 \u65b0\u3057\u3044shRNA\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3067\u8907\u6570\u8a66\u6599\u306e\u540c\u6642\u89e3\u6790\u3092\u53ef\u80fd\u306b 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[&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":66548,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[145,178],"class_list":["post-51305","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news","tag-pressrelease","tag-research"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts\/51305","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/comments?post=51305"}],"version-history":[{"count":1,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts\/51305\/revisions"}],"predecessor-version":[{"id":66549,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/posts\/51305\/revisions\/66549"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/media\/66548"}],"wp:attachment":[{"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/media?parent=51305"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/categories?post=51305"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.waseda.jp\/inst\/research\/wp-json\/wp\/v2\/tags?post=51305"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}