New method improving efficiency of shRNA screening

A group of researchers came up with a new method that improves the efficiency of short-hairpin RNA (shRNA) screening, which identifies biochemical interactions that produce pharmacological effects (known as mechanisms of action). Using this method, they found the gene that regulates cytotoxicity by aurilide B, a natural marine product that induces apoptosis, or programmed death of cells.

“Our new method using multiplex barcode sequencing technology facilitates the use of pooled shRNA library screening for the identification of combination drug therapy targets,” says Youichi Nakao, member of the research group and professor of biomolecular chemistry at Waseda University.

This research was published in Scientific Reports.

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